The Huntington's disease gene product, huntingtin, is indispensable for neural tube formation, but its role is obscure. We studied neurulation in htt-null embryonic stem cells and htt-morpholino zebrafish embryos and found a previously unknown, evolutionarily recent function for this ancient protein. We found that htt was essential for homotypic interactions between neuroepithelial cells; it permitted neurulation and rosette formation by regulating metalloprotease ADAM10 activity and Ncadherin cleavage. This function was embedded in the N terminus of htt and was phenocopied by treatment of htt knockdown zebrafish with an ADAM10 inhibitor. Notably, in htt-null cells, reversion of the rosetteless phenotype occurred only with expression of evolutionarily recent htt heterologues from deuterostome organisms. Conversely, all of the heterologues that we tested, including htt from Drosophila melanogaster and Dictyostelium discoideum, exhibited anti-apoptotic activity. Thus, anti-apoptosis may have been one of htt’s ancestral function(s), but, in deuterostomes, htt evolved to acquire a unique regulatory activity for controlling neural adhesion via ADAM10-Ncadherin, with implications for brain evolution and development.

An evolutionary recent neuroepithelial cell adhesion function of huntingtin implicates ADAM10-Ncadherin / V. Lo Sardo, C. Zuccato, G. Gaudenzi, B. Vitali, C. Ramos, M. Tartari, M.A. Myre, J.A. Walker, A. Pistocchi, L. Conti, M. Valenza, B. Drung, B. Schmidt, J. Gusella, S. Zeitlin, F. Cotelli, E. Cattaneo. - In: NATURE NEUROSCIENCE. - ISSN 1097-6256. - 15:5(2012 May), pp. 713-721. [10.1038/nn.3080]

An evolutionary recent neuroepithelial cell adhesion function of huntingtin implicates ADAM10-Ncadherin

V. Lo Sardo
Primo
;
C. Zuccato
Secondo
;
G. Gaudenzi;B. Vitali;A. Pistocchi;L. Conti;M. Valenza;F. Cotelli
Penultimo
;
E. Cattaneo
2012

Abstract

The Huntington's disease gene product, huntingtin, is indispensable for neural tube formation, but its role is obscure. We studied neurulation in htt-null embryonic stem cells and htt-morpholino zebrafish embryos and found a previously unknown, evolutionarily recent function for this ancient protein. We found that htt was essential for homotypic interactions between neuroepithelial cells; it permitted neurulation and rosette formation by regulating metalloprotease ADAM10 activity and Ncadherin cleavage. This function was embedded in the N terminus of htt and was phenocopied by treatment of htt knockdown zebrafish with an ADAM10 inhibitor. Notably, in htt-null cells, reversion of the rosetteless phenotype occurred only with expression of evolutionarily recent htt heterologues from deuterostome organisms. Conversely, all of the heterologues that we tested, including htt from Drosophila melanogaster and Dictyostelium discoideum, exhibited anti-apoptotic activity. Thus, anti-apoptosis may have been one of htt’s ancestral function(s), but, in deuterostomes, htt evolved to acquire a unique regulatory activity for controlling neural adhesion via ADAM10-Ncadherin, with implications for brain evolution and development.
wild-type huntingtin; disease gene homolog; embryonic stem-cells; N-cadherin cleavage; mutant huntingtin; targeted disruption; lacking huntingtin; ADAM10; mice; zebrafish
Settore BIO/14 - Farmacologia
Settore BIO/13 - Biologia Applicata
mag-2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/200397
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