A genotypic study using amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA fingerprinting (RAPD) and ribosomal spacer analysis (RSA) in comparison with DNA-DNA reassociation experiments was carried out with 85 thermophilic Bacillus isolates from uncultivated soil of 14 different geographical areas and seventeen reference strains representing defined thermophilic Bacillus species. This approach permitted the attribution of 51 % of the new isolates to the Bacillus thermoleovorans group and the identification of 40% of the new isolates as B. “thermodenitrificans”. Moreover, 2 strains were assigned to B. pallidus species and 1 isolate to B. thermosphaericus species. The remaining 6% of our thermophilic isolates from soil, constituting 2 DNA-DNA homology groups, are still unidentified. A detailed genotypic characterization of the heterogeneous species of B. thermoleovorans and B. stearothermophilus was also presented.
Genotypic characterization of thermophilic bacilli: a study on new soil isolates and several reference strains / D. Mora, M. Fortina, G. Nicastro, C. Parini, P. Manachini. - In: RESEARCH IN MICROBIOLOGY. - ISSN 0923-2508. - 149:10(1998), pp. 711-722.
|Titolo:||Genotypic characterization of thermophilic bacilli: a study on new soil isolates and several reference strains|
MORA, DIEGO (Primo)
FORTINA, MARIA GRAZIA (Secondo)
PARINI, CARLO (Penultimo)
MANACHINI, PIER LUIGI (Ultimo)
|Parole Chiave:||ARDRA-ITS; DNA-DNA homology; Identification; RAPD; RSA; Thermophilic Bacillus; ARDRA|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Data di pubblicazione:||1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0923-2508(99)80018-7|
|Appare nelle tipologie:||01 - Articolo su periodico|