The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group I of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the `B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the `B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.

16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16s rRNA group I of the genes Bacillus / D.G. Daffonchio, S. Borin, A. Consolandi, D. Mora, P.L. Manachini, C. Sorlini. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 163:2(1998), pp. 229-236.

16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16s rRNA group I of the genes Bacillus

D.G. Daffonchio
Primo
;
S. Borin
Secondo
;
D. Mora;P.L. Manachini
Penultimo
;
C. Sorlini
Ultimo
1998

Abstract

The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group I of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the `B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the `B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.
16S rRNA group 1; Bacillus; Identification; Internal transcribed spacer- polymerase chain reaction fingerprinting; Restriction analysis; Single-strand conformation polymorphism
Settore AGR/16 - Microbiologia Agraria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/196504
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