Microdialysis sampling and high performance liquid chromatography with amperometric detection for sugar analysis in milk products

A rapid and highly efficient method for the direct quantification of the major carbohydrates, glucose, fructose, sucrose and lactose in milk-based products is described. Rapid and selective monitoring of each sugar is accomplished by microdialysis sampling coupled with HPLC separation and amperometric detection with a chemically modified electrode. This new scheme offers simultaneous monitoring of sugars with little or no sample pretreatment. The results obtained from the application of the proposed procedure to milk and milk-based products are discussed.