Caseinphosphopeptides (CPPs) are peptides resulting from enzyme digestion of whole casein, characterized by the presence of an aminoacid cluster, negatively charged at physiological intestinal pH, that binds cations. The aim of the present study was to compare Ca absorption through the everted small intestine of rats from CaCl2 or Ca-CPPs solutions, in absence of inorganic phosphate (Pi) or when two ratios between Ca and Pi occur. Secondly, it was evaluated whether the ion absorption could be affected by the fatty acid pattern of enterocyte membrane. Results showed that in the absence of Pi, Ca transport from CaCl2 was higher than from Ca-CPPs solutions. However, when Pi was added to the CaCl2 solution, Ca absorption decreased by 90% (Ca:Pi = 1:1) and 97% (Ca:Pi = 1:2). On the contrary, when Pi was added to the Ca-CPPs solution, Ca transport decreased only by 40% (Ca:Pi = 1:1) and 60% (Ca:Pi = 1:2). The enrichment of enterocyte membrane with n-3 fatty acids (fish oil diet) compared to control diet did not modify the effect of CPPs on Ca transport in ileum of rats. These findings demonstrate that CPPs can limit the inhibitory effect of phosphate on Ca availability and increase Ca transport across the distal small intestine. This action could be of nutritional relevance especially when the dietary intake of this mineral is lower than the recommended level or there is an unbalanced ratio Ca/Pi in diet.

Effect of caseinphosphopeptides on inhibition of calcium intestinal absorption due to phosphate / D. Erba, S. Ciappellano, G. Testolin. - In: NUTRITION RESEARCH. - ISSN 0271-5317. - 21:4(2001), pp. 649-656. [10.1016/S0271-5317(01)00280-9]

Effect of caseinphosphopeptides on inhibition of calcium intestinal absorption due to phosphate

D. Erba;S. Ciappellano;G. Testolin
2001

Abstract

Caseinphosphopeptides (CPPs) are peptides resulting from enzyme digestion of whole casein, characterized by the presence of an aminoacid cluster, negatively charged at physiological intestinal pH, that binds cations. The aim of the present study was to compare Ca absorption through the everted small intestine of rats from CaCl2 or Ca-CPPs solutions, in absence of inorganic phosphate (Pi) or when two ratios between Ca and Pi occur. Secondly, it was evaluated whether the ion absorption could be affected by the fatty acid pattern of enterocyte membrane. Results showed that in the absence of Pi, Ca transport from CaCl2 was higher than from Ca-CPPs solutions. However, when Pi was added to the CaCl2 solution, Ca absorption decreased by 90% (Ca:Pi = 1:1) and 97% (Ca:Pi = 1:2). On the contrary, when Pi was added to the Ca-CPPs solution, Ca transport decreased only by 40% (Ca:Pi = 1:1) and 60% (Ca:Pi = 1:2). The enrichment of enterocyte membrane with n-3 fatty acids (fish oil diet) compared to control diet did not modify the effect of CPPs on Ca transport in ileum of rats. These findings demonstrate that CPPs can limit the inhibitory effect of phosphate on Ca availability and increase Ca transport across the distal small intestine. This action could be of nutritional relevance especially when the dietary intake of this mineral is lower than the recommended level or there is an unbalanced ratio Ca/Pi in diet.
Ca absorption; Caseinphosphopeptides; Phosphate
Settore BIO/09 - Fisiologia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/177627
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 44
  • ???jsp.display-item.citation.isi??? 33
social impact