The research was aimed at obtaining copper-enriched cells of Saccharomyces cerevisiae, developing an efficient metal delivery system for human nutrition and therapeutic treatments. To produce glutathione (GSH)–copper conjugates, four samples of commercial baker’s yeasts (S. cerevisiae) were employed in biotransformation trials with copper acetate. GSH production was found variable, ranging from 0.15 to 1.2% cell dw. Trials carried out with copper-adapted cells resulted in GSH and conjugate limited biosynthesis. The highest copper conjugate level (1.7% dw) was instead obtained in experiments set up by employing not adapted cells. Cell disruption by sonication and subsequent analysis by atomic absorption evidenced the presence of very interesting amount of intracellular copper, 1.8±0.2 mg/g celldw (i.e. 28 mol copper/g cell dw). The proposed procedure can be considered an interesting opportunity to further the range of application of yeast cells culture, for the set up of a copper delivery system for therapeutic treatments.

Process for obtaining copper-enriched cells of Saccharomyces cereviasiae / M. Rollini, A. Musatti, D. Erba, A. Benedetti, F. Girardo, M. Manzoni. - In: PROCESS BIOCHEMISTRY. - ISSN 1359-5113. - 46:7(2011), pp. 1417-1422.

Process for obtaining copper-enriched cells of Saccharomyces cereviasiae

M. Rollini
Primo
;
A. Musatti
Secondo
;
D. Erba;M. Manzoni
Ultimo
2011

Abstract

The research was aimed at obtaining copper-enriched cells of Saccharomyces cerevisiae, developing an efficient metal delivery system for human nutrition and therapeutic treatments. To produce glutathione (GSH)–copper conjugates, four samples of commercial baker’s yeasts (S. cerevisiae) were employed in biotransformation trials with copper acetate. GSH production was found variable, ranging from 0.15 to 1.2% cell dw. Trials carried out with copper-adapted cells resulted in GSH and conjugate limited biosynthesis. The highest copper conjugate level (1.7% dw) was instead obtained in experiments set up by employing not adapted cells. Cell disruption by sonication and subsequent analysis by atomic absorption evidenced the presence of very interesting amount of intracellular copper, 1.8±0.2 mg/g celldw (i.e. 28 mol copper/g cell dw). The proposed procedure can be considered an interesting opportunity to further the range of application of yeast cells culture, for the set up of a copper delivery system for therapeutic treatments.
Glutathione ; S. cerevisiae ; Copper conjugates ; Atomic absorption
Settore AGR/16 - Microbiologia Agraria
Settore BIO/09 - Fisiologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/175375
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