Green tea is considered as an aid to good health since it should increase the antioxidants defence system of the body against radical damage. The aim of this study was to investigate, in Jurkat cells, the protective effect of green tea extract (GTE) supplementation (10 mg/L, corresponding to 15 μmol/L EGCg, 24 h) against oxidative DNA damage induced by different concentrations of hydrogen peroxide (from 10 to 200 μmol/L H2O2 for 15 min). The DNA damage, evaluated by the Comet assay, was dose dependent, however it reached a plateau at 75 μmol/L of H2O2 without any protective effect exerted by GTE. The DNA repair process, completed within 2 hours, was unaffected by supplementation. These results seem to suggest that GTE supplementation, is not able to protect Jurkat cells against H2O2 induced DNA damage, at least under the conditions used in this study.
Effect of green tea extract on DNA repair and oxidative damage due to H2O2 in Jurkat T cells / P. Riso, D. Erba, F. Criscuoli, G. Testolin. - In: NUTRITION RESEARCH. - ISSN 0271-5317. - 22:10(2002), pp. 1143-1150.
|Titolo:||Effect of green tea extract on DNA repair and oxidative damage due to H2O2 in Jurkat T cells|
RISO, PATRIZIA (Primo)
ERBA, DANIELA (Secondo)
CRISCUOLI, FRANCA (Penultimo)
TESTOLIN, GIULIO (Ultimo)
|Parole Chiave:||Catechins; DNA damage; Green tea extract; Hydrogen peroxide; Jurkat T cells|
|Settore Scientifico Disciplinare:||Settore MED/49 - Scienze Tecniche Dietetiche Applicate|
Settore BIO/09 - Fisiologia
|Data di pubblicazione:||2002|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0271-5317(02)00424-4|
|Appare nelle tipologie:||01 - Articolo su periodico|