Green tea is considered as an aid to good health since it should increase the antioxidants defence system of the body against radical damage. The aim of this study was to investigate, in Jurkat cells, the protective effect of green tea extract (GTE) supplementation (10 mg/L, corresponding to 15 μmol/L EGCg, 24 h) against oxidative DNA damage induced by different concentrations of hydrogen peroxide (from 10 to 200 μmol/L H2O2 for 15 min). The DNA damage, evaluated by the Comet assay, was dose dependent, however it reached a plateau at 75 μmol/L of H2O2 without any protective effect exerted by GTE. The DNA repair process, completed within 2 hours, was unaffected by supplementation. These results seem to suggest that GTE supplementation, is not able to protect Jurkat cells against H2O2 induced DNA damage, at least under the conditions used in this study.

Effect of green tea extract on DNA repair and oxidative damage due to H2O2 in Jurkat T cells / P. Riso, D. Erba, F. Criscuoli, G. Testolin. - In: NUTRITION RESEARCH. - ISSN 0271-5317. - 22:10(2002), pp. 1143-1150. [10.1016/S0271-5317(02)00424-4]

Effect of green tea extract on DNA repair and oxidative damage due to H2O2 in Jurkat T cells

P. Riso;D. Erba;F. Criscuoli;G. Testolin
2002

Abstract

Green tea is considered as an aid to good health since it should increase the antioxidants defence system of the body against radical damage. The aim of this study was to investigate, in Jurkat cells, the protective effect of green tea extract (GTE) supplementation (10 mg/L, corresponding to 15 μmol/L EGCg, 24 h) against oxidative DNA damage induced by different concentrations of hydrogen peroxide (from 10 to 200 μmol/L H2O2 for 15 min). The DNA damage, evaluated by the Comet assay, was dose dependent, however it reached a plateau at 75 μmol/L of H2O2 without any protective effect exerted by GTE. The DNA repair process, completed within 2 hours, was unaffected by supplementation. These results seem to suggest that GTE supplementation, is not able to protect Jurkat cells against H2O2 induced DNA damage, at least under the conditions used in this study.
Catechins; DNA damage; Green tea extract; Hydrogen peroxide; Jurkat T cells
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Settore BIO/09 - Fisiologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/166967
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