Proteomic studies on the protein fraction of germinating legume seeds exudates revea- led that during the first hours from imbibition white lupin seeds selectively release a variety of polypeptides into the germinating medium. Class-III chitinase is the most represented protein, accounting for more than 33%. The release of this protein lasts for at least three days upon seed imbibition. The physiological meaning of this massive flow of chitinase outside the seeds is still unknown. RT-qPCR analysis and pulse-chase experiments indicated that the protein is de novo synthesized from mature seed stored mRNA and that no new mRNA synthesis occurs during germination. Instead, when seeds were germinated in the presence of jasmonic acid and chitosan a strong boost of expression was observed, supporting a direct defence role of the protein. Apart the inhibition of the growth of fungi through the hydrolysis of cell wall chitin, it has been postulated that the role of chitinases in healthy plant is to generate signal molecules. These molecules are not only involved in plant resistance to external environmental factors but also in plant growth and development events, such as host specificity of Rhizobia, nodule development and process of cold acclimatation. By combining the results obtained using different experimental approaches, the aim of the present work is to contribute to unveil the role of class-III chitinase in germinating lupin seeds.

Massive secretion of de novo-synthesized chitinase during germination : a defense role only? / A. Scarafoni, A. Ronchi, B. Prinsi, L. Espen, A. Consonni, M. Duranti. ((Intervento presentato al 13. convegno International Lupin Conference tenutosi a Poznam nel 2011.

Massive secretion of de novo-synthesized chitinase during germination : a defense role only?

A. Scarafoni
Primo
;
B. Prinsi;L. Espen;A. Consonni
Penultimo
;
M. Duranti
Ultimo
2011

Abstract

Proteomic studies on the protein fraction of germinating legume seeds exudates revea- led that during the first hours from imbibition white lupin seeds selectively release a variety of polypeptides into the germinating medium. Class-III chitinase is the most represented protein, accounting for more than 33%. The release of this protein lasts for at least three days upon seed imbibition. The physiological meaning of this massive flow of chitinase outside the seeds is still unknown. RT-qPCR analysis and pulse-chase experiments indicated that the protein is de novo synthesized from mature seed stored mRNA and that no new mRNA synthesis occurs during germination. Instead, when seeds were germinated in the presence of jasmonic acid and chitosan a strong boost of expression was observed, supporting a direct defence role of the protein. Apart the inhibition of the growth of fungi through the hydrolysis of cell wall chitin, it has been postulated that the role of chitinases in healthy plant is to generate signal molecules. These molecules are not only involved in plant resistance to external environmental factors but also in plant growth and development events, such as host specificity of Rhizobia, nodule development and process of cold acclimatation. By combining the results obtained using different experimental approaches, the aim of the present work is to contribute to unveil the role of class-III chitinase in germinating lupin seeds.
2011
Settore BIO/10 - Biochimica
International Lupin Association
Massive secretion of de novo-synthesized chitinase during germination : a defense role only? / A. Scarafoni, A. Ronchi, B. Prinsi, L. Espen, A. Consonni, M. Duranti. ((Intervento presentato al 13. convegno International Lupin Conference tenutosi a Poznam nel 2011.
Conference Object
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/159602
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact