Aim. In maize Brachytic 2 (Br2) gene encodes for an auxin polar transporter similar to adenosine triphosphate (ATP)-binding cassette transporter of the multidrug resistant (MDR) class of P-glycopoteins (PGPs) protein family. Mutations in Br2 gene cause a reduction in plant stature (about 50%) due to shortening of the internodes length without a corresponding reduction in the number of internodes or in the size of other organs. Furthermore br2 mutation confers several positive agronomic traits such as stay green, resistance to lodging and in general a modified plant architecture mimicking modern hybrids. The aim of this work is to evaluate a possible application of br2 mutation as heterozygous (Br2/br2) in maize genetic improvement (in fact br2 mutation exhibits a phenomenon of incomplete dominance). Methods. With this purpose we constituted several hybrids differing only for br2 constitution (Br2/Br2 vs Br2/br2) and we compared some agronomic traits, such as plant height and ear height. We supported the breeding activity using a Sequence-Specific Amplification Polymorphism (S-SAP) molecular marker to confirm the presence of br2-23 mutation in the genetic material constituted. Results. The results obtained showed an useful effect of this mutation in some of the genetic backgrounds studied. Conclusion. Further breeding programs are necessary to explore the full potential of this new finding.

Evaluation of a possible use of brachytic 2 mutation in corn breeding / D. Villa, F. Cerino Badone, A. Bucci, E. Cassani, R. Pilu. - In: MINERVA BIOTECNOLOGICA. - ISSN 1120-4826. - 22:2(2010), pp. 59-62.

Evaluation of a possible use of brachytic 2 mutation in corn breeding

F. Cerino Badone;A. Bucci;E. Cassani;R. Pilu
2010

Abstract

Aim. In maize Brachytic 2 (Br2) gene encodes for an auxin polar transporter similar to adenosine triphosphate (ATP)-binding cassette transporter of the multidrug resistant (MDR) class of P-glycopoteins (PGPs) protein family. Mutations in Br2 gene cause a reduction in plant stature (about 50%) due to shortening of the internodes length without a corresponding reduction in the number of internodes or in the size of other organs. Furthermore br2 mutation confers several positive agronomic traits such as stay green, resistance to lodging and in general a modified plant architecture mimicking modern hybrids. The aim of this work is to evaluate a possible application of br2 mutation as heterozygous (Br2/br2) in maize genetic improvement (in fact br2 mutation exhibits a phenomenon of incomplete dominance). Methods. With this purpose we constituted several hybrids differing only for br2 constitution (Br2/Br2 vs Br2/br2) and we compared some agronomic traits, such as plant height and ear height. We supported the breeding activity using a Sequence-Specific Amplification Polymorphism (S-SAP) molecular marker to confirm the presence of br2-23 mutation in the genetic material constituted. Results. The results obtained showed an useful effect of this mutation in some of the genetic backgrounds studied. Conclusion. Further breeding programs are necessary to explore the full potential of this new finding.
Genes, plant; Plant structures; Zea mays
Settore AGR/07 - Genetica Agraria
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/150739
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 1
social impact