The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.
Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34(+)38(-) compartment / L. Pedranzini, F. Mottadelli, S. Ronzoni, F. Rossella, M. Ferracin, I. Magnani, G. Roversi, P. Colapietro, M. Negrini, P.G. Pelicci, L. Larizza. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 34:10(2010 Oct), pp. 1287-1295.
Titolo: | Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34(+)38(-) compartment |
Autori: | PEDRANZINI, LAURA (Primo) MOTTADELLI, FEDERICA (Secondo) PELICCI, PIER GIUSEPPE (Penultimo) LARIZZA, LIDIA (Ultimo) |
Parole Chiave: | AML; CD34+CD38-; Cytogenomic characterization; Kasumi-1; MiRNA expression |
Settore Scientifico Disciplinare: | Settore MED/04 - Patologia Generale Settore MED/03 - Genetica Medica |
Data di pubblicazione: | ott-2010 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.leukres.2010.02.012 |
Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
File | Descrizione | Tipologia | Licenza | |
---|---|---|---|---|
14001623165602715.pdf | Publisher's version/PDF | UNIVERSITY_NETWORK Richiedi una copia |