The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.

Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34(+)38(-) compartment / L. Pedranzini, F. Mottadelli, S. Ronzoni, F. Rossella, M. Ferracin, I. Magnani, G. Roversi, P. Colapietro, M. Negrini, P.G. Pelicci, L. Larizza. - In: LEUKEMIA RESEARCH. - ISSN 0145-2126. - 34:10(2010 Oct), pp. 1287-1295. [10.1016/j.leukres.2010.02.012]

Differential cytogenomics and miRNA signature of the Acute Myeloid Leukaemia Kasumi-1 cell line CD34(+)38(-) compartment

L. Pedranzini
Primo
;
F. Mottadelli
Secondo
;
F. Rossella;I. Magnani;G. Roversi;P. Colapietro;P.G. Pelicci
Penultimo
;
L. Larizza
Ultimo
2010

Abstract

The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34(+)CD38(-) fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34(+)CD38(-) cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34(+)CD38(-) fraction.
AML; CD34+CD38-; Cytogenomic characterization; Kasumi-1; MiRNA expression
Settore MED/04 - Patologia Generale
Settore MED/03 - Genetica Medica
ott-2010
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/148858
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