AIM: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns. METHODS: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence) and the tight-junction protein occludin and zonula occluden-1. RESULTS: The treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin fi laments, impaired and disassembled the integrity of the tight-junction system. CONCLUSION: Our data obtained from an "in vivo - like" polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions.

Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model / E. Dolfini, L.R. Roncoroni, Luca Elli, Chiara Fumagalli, Roberto Colombo, Simona Ramponi, Fabio Forlani, M.T. Bardella. - In: WORLD JOURNAL OF GASTROENTEROLOGY. - ISSN 1007-9327. - 11:48(2005), pp. 7597-7601.

Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model

E. Dolfini
Primo
;
L.R. Roncoroni
Secondo
;
L. Elli;R. Colombo;F. Forlani;M.T. Bardella
Ultimo
2005

Abstract

AIM: To evaluate the interplay between gliadin and LoVo cells and the direct effect of gliadin on cytoskeletal patterns. METHODS: We treated LoVo multicellular spheroids with digested bread wheat gliadin in order to investigate their morphology and ultrastructure (by means of light microscopy and scanning electron microscopy), and the effect of gliadin on actin (phalloidin fluorescence) and the tight-junction protein occludin and zonula occluden-1. RESULTS: The treated spheroids had deep holes and surface blebs, whereas the controls were smoothly surfaced ovoids. The incubation of LoVo spheroids with gliadin decreased the number of intracellular actin fi laments, impaired and disassembled the integrity of the tight-junction system. CONCLUSION: Our data obtained from an "in vivo - like" polarized culture system confirm the direct noxious effect of gliadin on the cytoskeleton and tight junctions of epithelial cells. Unlike two-dimensional cell culture systems, the use of multicellular spheroids seems to provide a suitable model for studying cell-cell interactions.
Celiac disease; Cytoskeleton; Gliadin; Multicellular spheroids
Settore BIO/10 - Biochimica
Settore BIO/13 - Biologia Applicata
Settore BIO/06 - Anatomia Comparata e Citologia
Settore MED/12 - Gastroenterologia
http://www.wjgnet.com/1007-9327/11/7597.asp
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/13074
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