Supplementing cattle diets with tannins is a strategy to decrease enteric methane (CH4) production, mitigating the environmental impact of livestock production. In the present study, a 10-day rumen simulation technique (Rusitec) was used to evaluate the effect of two hydrolysable tannin metabolites, gallic acid (GA) and ellagic acid (EA), and their combination on rumen microbiota. The EA treatments significantly reduced daily CH4 production by up to 60%. The qPCR showed a reduction of the fibrolytic bacteria Butyrivibrio fibrisolvens, Fibrobacter succinogenes and Ruminococcus flavefaciens as well as an increase of the nitrate-reducing Selenomonas ruminantium after EA treatment, thus modulating feed fermentation and in turn methanogenesis. Amplicon sequencing revealed a stronger impact of EA on bacteria than archaea, with reduced bacterial alpha-diversity. Bacterial communities were dominated by Megasphera elsdenii after EA treatments, while archaeal communities were always dominated by the Methanomethylophilaceae family. Therefore, EA rather than GA increased the abundance of M. elsdenii, likely redirecting H2 from methanogenesis to other H2 sinks. However, EA in contrast to GA also induced a decrease of short-chain fatty acids production and nutrient degradation. Overall, EA addition induced stronger effects compared to GA. This underlines the need to assess effects of individual tannin components on rumen processes to develop efficient CH4 mitigation strategies.
Effects of hydrolysable tannin metabolites on rumen microbiota and enteric methane production / M. Manoni, F. Gschwend, S. Amelchanka, M. Terranova, L. Pinotti, F. Widmer, P. Silacci, M. Tretola - In: Book of Abstracts of the 75th Annual Meeting of the European Federation of Animal SciencePrima edizione. - [s.l] : EAAP Scientific Committe, 2024 Aug. - ISBN 979-12-210-6769-9. - pp. 564-564 (( Intervento presentato al 75. convegno Annual Meeting of the European Federation of Animal Science tenutosi a Firenze nel 2024.
Effects of hydrolysable tannin metabolites on rumen microbiota and enteric methane production
M. Manoni;L. Pinotti;
2024
Abstract
Supplementing cattle diets with tannins is a strategy to decrease enteric methane (CH4) production, mitigating the environmental impact of livestock production. In the present study, a 10-day rumen simulation technique (Rusitec) was used to evaluate the effect of two hydrolysable tannin metabolites, gallic acid (GA) and ellagic acid (EA), and their combination on rumen microbiota. The EA treatments significantly reduced daily CH4 production by up to 60%. The qPCR showed a reduction of the fibrolytic bacteria Butyrivibrio fibrisolvens, Fibrobacter succinogenes and Ruminococcus flavefaciens as well as an increase of the nitrate-reducing Selenomonas ruminantium after EA treatment, thus modulating feed fermentation and in turn methanogenesis. Amplicon sequencing revealed a stronger impact of EA on bacteria than archaea, with reduced bacterial alpha-diversity. Bacterial communities were dominated by Megasphera elsdenii after EA treatments, while archaeal communities were always dominated by the Methanomethylophilaceae family. Therefore, EA rather than GA increased the abundance of M. elsdenii, likely redirecting H2 from methanogenesis to other H2 sinks. However, EA in contrast to GA also induced a decrease of short-chain fatty acids production and nutrient degradation. Overall, EA addition induced stronger effects compared to GA. This underlines the need to assess effects of individual tannin components on rumen processes to develop efficient CH4 mitigation strategies.File | Dimensione | Formato | |
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