Tissue fluidification and collective motility are pivotal in regulating embryonic morphogenesis, wound healing, and tumor metastasis. These processes frequently require that each cell constituent of a tissue coordinates its migration activity and directed motion through the oriented extension of lamellipodium cell protrusions, promoted by RAC1 activity. While the upstream RAC1 regulators in individual migratory cells or leader cells during invasion or wound healing are well characterized, how RAC1 is controlled in follower cells remains unknown. Here, we identify a MYO6-DOCK7 axis essential for spatially restricting RAC1 activity in a planar polarized fashion in model tissue monolayers. The MYO6-DOCK7 axis specifically controls the extension of cryptic lamellipodia required to drive tissue fluidification and cooperative-mode motion in otherwise solid and static carcinoma cell collectives.

A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epithelia / L. Menin, J. Weber, S. Villa, E. Martini, E. Maspero, C.A. Niño, V. Cancila, A. Poli, P. Maiuri, A. Palamidessi, E. Frittoli, F. Bianchi, C. Tripodo, K.J. Walters, F. Giavazzi, G. Scita, S. Polo. - In: CELL REPORTS. - ISSN 2211-1247. - 42:8(2023 Aug 29), pp. 113001.1-113001.16. [10.1016/j.celrep.2023.113001]

A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epithelia

L. Menin
Primo
Writing – Review & Editing
;
S. Villa;E. Martini;E. Maspero;A. Poli;A. Palamidessi;F. Bianchi;F. Giavazzi;G. Scita
Penultimo
;
S. Polo
Ultimo
2023

Abstract

Tissue fluidification and collective motility are pivotal in regulating embryonic morphogenesis, wound healing, and tumor metastasis. These processes frequently require that each cell constituent of a tissue coordinates its migration activity and directed motion through the oriented extension of lamellipodium cell protrusions, promoted by RAC1 activity. While the upstream RAC1 regulators in individual migratory cells or leader cells during invasion or wound healing are well characterized, how RAC1 is controlled in follower cells remains unknown. Here, we identify a MYO6-DOCK7 axis essential for spatially restricting RAC1 activity in a planar polarized fashion in model tissue monolayers. The MYO6-DOCK7 axis specifically controls the extension of cryptic lamellipodia required to drive tissue fluidification and cooperative-mode motion in otherwise solid and static carcinoma cell collectives.
CP: Cell biology
Settore BIO/11 - Biologia Molecolare
   Impact of cell shapes on cell behaviour and fate (SHAPINCELLFATE)
   SHAPINCELLFATE
   EUROPEAN COMMISSION
   101071470

   Dipartimenti di Eccellenza 2018-2022 - Dipartimento di ONCOLOGIA ED EMATO-ONCOLOGIA
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
29-ago-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1026648
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