The conjugation of tetraphenylethylene (TPE) with podophyllotoxin, N-desacetyl-thiocolchicine, and cabazitaxel through a sebacic acid linker led to the formation of fluorescent nanoparticles. Dynamic light scattering (DLS) and photoluminescence spectroscopy were used for the identification and characterization of the fluorescent nanoparticles. The biological evaluation was determined in three human ovarian (KURAMOCHI, OVCAR3, OVSAHO) and three human breast (MCF7, SKBR 3, and MDA-MB231) cancer cell lines. In the case of cabazitaxel, the nanoparticles maintained the activity of the parent drug, at the low nanomolar range, while exhibiting high blue fluorescence. The internalization of the fluorescent NPs into cells was detected using immunofluorescence assay.
Tetraphenylethylene-Based Photoluminescent Self-Assembled Nanoparticles: Preparation and Biological Evaluation / E. Colombo, E.M. Aydın, İ.S. Canıtez, L. Polito, M. Penconi, A. Bossi, E. Impresari, D. Passarella, S. Dallavalle, C.M. Athanassopoulos, S. Pellegrino, I.D. Şahin, M.S. Christodoulou. - In: ACS MEDICINAL CHEMISTRY LETTERS. - ISSN 1948-5875. - 14:10(2023 Oct 12), pp. 1472-1477. [10.1021/acsmedchemlett.3c00396]
Tetraphenylethylene-Based Photoluminescent Self-Assembled Nanoparticles: Preparation and Biological Evaluation
L. Polito;A. Bossi;E. Impresari;D. Passarella;S. Dallavalle;S. Pellegrino;M.S. Christodoulou
Ultimo
2023
Abstract
The conjugation of tetraphenylethylene (TPE) with podophyllotoxin, N-desacetyl-thiocolchicine, and cabazitaxel through a sebacic acid linker led to the formation of fluorescent nanoparticles. Dynamic light scattering (DLS) and photoluminescence spectroscopy were used for the identification and characterization of the fluorescent nanoparticles. The biological evaluation was determined in three human ovarian (KURAMOCHI, OVCAR3, OVSAHO) and three human breast (MCF7, SKBR 3, and MDA-MB231) cancer cell lines. In the case of cabazitaxel, the nanoparticles maintained the activity of the parent drug, at the low nanomolar range, while exhibiting high blue fluorescence. The internalization of the fluorescent NPs into cells was detected using immunofluorescence assay.File | Dimensione | Formato | |
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ACS Med. Chem. Lett. 2023, 14, 1472−1477.pdf
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