Conversion of plasminogen to plasmin provides an important source of proteolytic activity in the bovine mammary gland. Plasminogen activator inhibitor 1 (PAI-1) plays a key role in limiting plasminogen activation. The PAI-1 biosynthetic capabilities of various bovine mammary cells were determined. The immortalized epithelial cell lines MAC-T and BME-UV and the myoepithelial cell line BMM-UV were used as model systems. Northern blot analysis indicated that both epithelial and myoepithelial cells contained PAI-1 mRNA. Bovine PAI-1 was encoded by a single mRNA species ∼ 3·0 kb long. BME-UV cells contained 2·0-fold (P < 0·01) the PAI-1 mRNA of MAC-T or BMM-UV cells. Reverse zymography indicated that both epithelial and myoepithelial cells synthesized PAI-1 protein with a molecular mass of ∼ 50 kDa.
Synthesis of plasminogen activator inhibitor 1 by bovine mammary epithelial and myoepithelial cell lines / B. Zavizion, C.W. Heegard, J. White, F. Cheli, I. Politis. - In: THE JOURNAL OF DAIRY RESEARCH. - ISSN 0022-0299. - 63:3(1996 Aug), pp. 451-458. [10.1017/s0022029900031952]
Synthesis of plasminogen activator inhibitor 1 by bovine mammary epithelial and myoepithelial cell lines
F. CheliPenultimo
;
1996
Abstract
Conversion of plasminogen to plasmin provides an important source of proteolytic activity in the bovine mammary gland. Plasminogen activator inhibitor 1 (PAI-1) plays a key role in limiting plasminogen activation. The PAI-1 biosynthetic capabilities of various bovine mammary cells were determined. The immortalized epithelial cell lines MAC-T and BME-UV and the myoepithelial cell line BMM-UV were used as model systems. Northern blot analysis indicated that both epithelial and myoepithelial cells contained PAI-1 mRNA. Bovine PAI-1 was encoded by a single mRNA species ∼ 3·0 kb long. BME-UV cells contained 2·0-fold (P < 0·01) the PAI-1 mRNA of MAC-T or BMM-UV cells. Reverse zymography indicated that both epithelial and myoepithelial cells synthesized PAI-1 protein with a molecular mass of ∼ 50 kDa.
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