The Programmed Death-Ligand 1 (PD-L1) protein, belonging to the B7 family, is highly expressed in various tumours. Therefore, its early detection plays a significant role in cancer diagnostics and treatment1. Various methods have been used and investigated to identify the protein accurately. Among them, Enzyme-Linked Immunosorbent Assay (ELISA) is the gold-standard technique. However, it is laborious and requires a relatively long assay time. Herein, we developed a highly sensitive electrochemical biosensor for PDL-1 detection as a non-invasive biomarker for the early diagnosis of most cancers. The proposed platform is based on the deposition of gold nanoislands on screen-printed carbon electrodes by flame spray pyrolysis method2. The electrochemical responses of the proposed biosensors were investigated via cyclic voltammetry and differential pulse voltammetry. The proposed platform demonstrated an excellent linear response towards PDL-1 in the range of concentrations from sub-micromolar to attomolar. Moreover, the biosensor was found to manifest desirable selectivity towards the target analyte in the presence of other proteins and biomolecules in blood serum. The results are geared toward paving the way for the successful implementation of point-of- the-care devices for PDL-1 detection, allowing rapid and accurate diagnosis of early stages of cancer.
Label-free electrochemical detection of the cancer biomarker PDL-1 protein / Z. Lotfibakalani, M. Ghosh Dastidar, T. Tran-Phu, K. Murugappan, B. Karawdeniya, E. Pargoletti, P. Moazzam, A. Damry, D. Nisbet, A. Tricoli. ((Intervento presentato al 23. convegno Australasian Electrochemistry Symposium tenutosi a Griffith : 7 May nel 2023.
Label-free electrochemical detection of the cancer biomarker PDL-1 protein
E. Pargoletti;
2023
Abstract
The Programmed Death-Ligand 1 (PD-L1) protein, belonging to the B7 family, is highly expressed in various tumours. Therefore, its early detection plays a significant role in cancer diagnostics and treatment1. Various methods have been used and investigated to identify the protein accurately. Among them, Enzyme-Linked Immunosorbent Assay (ELISA) is the gold-standard technique. However, it is laborious and requires a relatively long assay time. Herein, we developed a highly sensitive electrochemical biosensor for PDL-1 detection as a non-invasive biomarker for the early diagnosis of most cancers. The proposed platform is based on the deposition of gold nanoislands on screen-printed carbon electrodes by flame spray pyrolysis method2. The electrochemical responses of the proposed biosensors were investigated via cyclic voltammetry and differential pulse voltammetry. The proposed platform demonstrated an excellent linear response towards PDL-1 in the range of concentrations from sub-micromolar to attomolar. Moreover, the biosensor was found to manifest desirable selectivity towards the target analyte in the presence of other proteins and biomolecules in blood serum. The results are geared toward paving the way for the successful implementation of point-of- the-care devices for PDL-1 detection, allowing rapid and accurate diagnosis of early stages of cancer.
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