The transformation of meso-butane-2,3-diol into butan-2-ol by a strain of Lactobacillus brevis occurs through a diol-dehydratase-catalyzed conversion of the diol into butan-2-one, which is then reduced to the secondary alcohol by dehydrogenases. Experiments performed with deuterated meso-butane-2,3-diols showed that the dehydration reaction brings about an inversion of configuration at the (R)-configured C-atom of mesobutane-2,3-diol as a consequence of the substitution of the OH group by a H-atom; at the same time, the H-atom already bound to the (R) C-atom is retained in the resulting methylene group. The H-atom replacing the OH group was assessed to come from the medium since the H-atom at the (S)-configured C-atom was completely lost. By contrast, in the case of the conversion of (RS)-propane-1,2-diol into propan-1-ol under the same fermentation conditions, an extensive H-transfer (ca. 80%) from the primary-alcohol function to the adjacent C-atom was observed. This fact is taken as an indication of different modes in which the two substrates are processed by the enzyme (despite the same stereochemical outcome). A speculative hypothesis is presented to interpret such a dissimilarity.
Stereochemistry and fate of hydrogen atoms in the diol-dehydratase-catalyzed dehydration of meso-butane-2,3-diol / P. Manitto, G. Speranza, G. Fontana, A. Galli. - In: HELVETICA CHIMICA ACTA. - ISSN 0018-019X. - 81:11(1998 Nov), pp. 2005-2016. [10.1002/(SICI)1522-2675(19981111)81:11<2005::AID-HLCA2005>3.3.CO;2-U]
Stereochemistry and fate of hydrogen atoms in the diol-dehydratase-catalyzed dehydration of meso-butane-2,3-diol
P. Manitto
Primo
;G. SperanzaSecondo
;A. GalliUltimo
1998
Abstract
The transformation of meso-butane-2,3-diol into butan-2-ol by a strain of Lactobacillus brevis occurs through a diol-dehydratase-catalyzed conversion of the diol into butan-2-one, which is then reduced to the secondary alcohol by dehydrogenases. Experiments performed with deuterated meso-butane-2,3-diols showed that the dehydration reaction brings about an inversion of configuration at the (R)-configured C-atom of mesobutane-2,3-diol as a consequence of the substitution of the OH group by a H-atom; at the same time, the H-atom already bound to the (R) C-atom is retained in the resulting methylene group. The H-atom replacing the OH group was assessed to come from the medium since the H-atom at the (S)-configured C-atom was completely lost. By contrast, in the case of the conversion of (RS)-propane-1,2-diol into propan-1-ol under the same fermentation conditions, an extensive H-transfer (ca. 80%) from the primary-alcohol function to the adjacent C-atom was observed. This fact is taken as an indication of different modes in which the two substrates are processed by the enzyme (despite the same stereochemical outcome). A speculative hypothesis is presented to interpret such a dissimilarity.File | Dimensione | Formato | |
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Helvetica Chimica Acta - 1998 - Manitto - Stereochemistry and Fate of Hydrogen Atoms in the Diol‐Dehydratase‐Catalyzed.pdf
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