BAP1 is mutated or deleted in many cancer types, including mesothelioma, uveal melanoma, and cholangiocarcinoma. It is the catalytic subunit of the PR-DUB complex, which removes PRC1-mediated H2AK119ub1, essential for maintaining transcriptional repression. However, the precise relationship between BAP1 and Polycombs remains elusive. Using embryonic stem cells, we show that BAP1 restricts H2AK119ub1 deposition to Polycomb target sites. This increases the stability of Polycomb with their targets and prevents diffuse accumulation of H2AK119ub1 and H3K27me3. Loss of BAP1 results in a broad increase in H2AK119ub1 levels that is primarily dependent on PCGF3/5-PRC1 complexes. This titrates PRC2 away from its targets and stimulates H3K27me3 accumulation across the genome, leading to a general chromatin compaction. This provides evidence for a unifying model that resolves the apparent contradiction between BAP1 catalytic activity and its role in vivo, uncovering molecular vulnerabilities that could be useful for BAP1-related pathologies.

BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation / E. Conway, F. Rossi, D. Fernandez-Perez, E. Ponzo, K.J. Ferrari, M. Zanotti, D. Manganaro, S. Rodighiero, S. Tamburri, D. Pasini. - In: MOLECULAR CELL. - ISSN 1097-2765. - 81:17(2021 Sep 02), pp. 3526-3541. [10.1016/j.molcel.2021.06.020]

BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation

D. Fernandez-Perez;E. Ponzo;K.J. Ferrari;S. Rodighiero;S. Tamburri;D. Pasini
Ultimo
2021

Abstract

BAP1 is mutated or deleted in many cancer types, including mesothelioma, uveal melanoma, and cholangiocarcinoma. It is the catalytic subunit of the PR-DUB complex, which removes PRC1-mediated H2AK119ub1, essential for maintaining transcriptional repression. However, the precise relationship between BAP1 and Polycombs remains elusive. Using embryonic stem cells, we show that BAP1 restricts H2AK119ub1 deposition to Polycomb target sites. This increases the stability of Polycomb with their targets and prevents diffuse accumulation of H2AK119ub1 and H3K27me3. Loss of BAP1 results in a broad increase in H2AK119ub1 levels that is primarily dependent on PCGF3/5-PRC1 complexes. This titrates PRC2 away from its targets and stimulates H3K27me3 accumulation across the genome, leading to a general chromatin compaction. This provides evidence for a unifying model that resolves the apparent contradiction between BAP1 catalytic activity and its role in vivo, uncovering molecular vulnerabilities that could be useful for BAP1-related pathologies.
BAP1; H2AK119ub1; PCGF3; PR-DUB; PRC1; PRC2; Polycomb; chromatin compaction; transcriptional repression; tumor suppressor; Animals; Cell Line; Chromatin; Embryonic Stem Cells; Heterochromatin; Histones; Humans; Mice; Mouse Embryonic Stem Cells; Polycomb Repressive Complex 1; Polycomb Repressive Complex 2; Polycomb-Group Proteins; Tumor Suppressor Proteins; Ubiquitin Thiolesterase; Ubiquitination
Settore BIO/11 - Biologia Molecolare
28-giu-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946749
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