Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.

Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality / N. Zaghet, K. Madsen, F. Rossi, D.F. Perez, P.G. Amendola, S. Demharter, U. Pfisterer, K. Khodosevich, D. Pasini, A.E. Salcini. - In: CELL REPORTS. - ISSN 2211-1247. - 37:8(2021), pp. 110050.1-110050.15.e6. [10.1016/j.celrep.2021.110050]

Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality

F. Rossi;D. Pasini
Penultimo
;
2021

Abstract

Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.
English
germ cell identity; germ cell immortality; H3K27 methylation; H3K36 methylation; histone demethylases; temperature
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
   Dissecting the Function of Multiple Polycomb Group Complexes in Establishing Transcriptional Identity
   DissectPcG
   European Commission
   Horizon 2020 Framework Programme
   725268
2021
Elsevier B.V.
37
8
110050
1
15.e6
15
Pubblicato
Periodico con rilevanza internazionale
scopus
orcid
crossref
wos
Aderisco
info:eu-repo/semantics/article
Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality / N. Zaghet, K. Madsen, F. Rossi, D.F. Perez, P.G. Amendola, S. Demharter, U. Pfisterer, K. Khodosevich, D. Pasini, A.E. Salcini. - In: CELL REPORTS. - ISSN 2211-1247. - 37:8(2021), pp. 110050.1-110050.15.e6. [10.1016/j.celrep.2021.110050]
open
Prodotti della ricerca::01 - Articolo su periodico
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Article (author)
Periodico con Impact Factor
N. Zaghet, K. Madsen, F. Rossi, D.F. Perez, P.G. Amendola, S. Demharter, U. Pfisterer, K. Khodosevich, D. Pasini, A.E. Salcini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946616
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