2-chloro-adenosine induced apoptosis of astroglial cells in rat brain cultures, as shown by flow cytometry and morphological analysis. The adenosine analogue was far more potent than several previously characterized sugars (including 2-deoxy-D-ribose and D-ribose, the sugar moiety of 2-chloro-adenosine), which trigger apoptosis in a variety of cell-lines [8-10], suggesting that the effects of 2-chloro-adenosine are only partially dictated by its sugar moiety. Nevertheless, 2-chloro-adenosine and 2-deoxy-D-ribose attenuated each other's cell death when used in combination, suggesting the involvement of common intracellular mechanisms. It is suggested that 2-chloro-adenosine may induce apoptosis via a yet-to-be identified adenosine receptor, which may have intriguing implications for both nervous system development and brain response to trauma and ischemia. (C) 1995 Academic Press, Inc.

A Novel Action for Adenosine: Apoptosis of Astroglial Cells in Rat-Brain Primary Cultures / M.P. Abbracchio, S. Ceruti, D. Barbieri, C. Franceschi, W. Malorni, L. Biondo, G. Burnstock, F. Cattabeni. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 213:3(1995 Aug 24), pp. 908-915. [10.1006/bbrc.1995.2215]

A Novel Action for Adenosine: Apoptosis of Astroglial Cells in Rat-Brain Primary Cultures

M.P. Abbracchio
Primo
;
S. Ceruti
Secondo
;
F. Cattabeni
1995

Abstract

2-chloro-adenosine induced apoptosis of astroglial cells in rat brain cultures, as shown by flow cytometry and morphological analysis. The adenosine analogue was far more potent than several previously characterized sugars (including 2-deoxy-D-ribose and D-ribose, the sugar moiety of 2-chloro-adenosine), which trigger apoptosis in a variety of cell-lines [8-10], suggesting that the effects of 2-chloro-adenosine are only partially dictated by its sugar moiety. Nevertheless, 2-chloro-adenosine and 2-deoxy-D-ribose attenuated each other's cell death when used in combination, suggesting the involvement of common intracellular mechanisms. It is suggested that 2-chloro-adenosine may induce apoptosis via a yet-to-be identified adenosine receptor, which may have intriguing implications for both nervous system development and brain response to trauma and ischemia. (C) 1995 Academic Press, Inc.
2-Chloroadenosine; Animals; Apoptosis; Astrocytes; Cells, Cultured; Corpus Striatum; Deoxyribose; Enzyme Activation; Poly(ADP-ribose) Polymerases; Rats; Rats, Sprague-Dawley; Structure-Activity Relationship;
Settore BIO/14 - Farmacologia
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0006291X85722152-main.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 505.29 kB
Formato Adobe PDF
505.29 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946364
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 82
  • ???jsp.display-item.citation.isi??? 81
social impact