Although much is known about seed and fruit development at the molecular level, many gaps remain in our understanding of how cell wall modifications can impact developmental processes in plants, as well as how biomechanical alterations influence seed and fruit growth. Mutants of Arabidopsis thaliana constitute an excellent tool to study the function of gene families devoted to cell wall biogenesis. We have characterized a collection of lines carrying mutations in representative cell wall-related genes for seed and fruit size developmental defects, as well as altered germination rates. We have linked these studies to cell wall composition and structure. Interestingly, we have found that disruption of genes involved in pectin maturation and hemicellulose deposition strongly influence germination dynamics. Finally, we focused on two transcriptional regulators, SEEDSTICK (STK) and LEUNIG-HOMOLOG (LUH), which positively regulate seed growth. Herein, we demonstrate that these factors regulate specific aspects of cell wall properties such as pectin distribution. We propose a model wherein changes in seed coat structure due to alterations in the xyloglucan-cellulose matrix deposition and pectin maturation are critical for organ growth and germination. The results demonstrate the importance of cell wall properties and remodeling of polysaccharides as major factors responsible for seed development.

The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control / M. Di Marzo, N. Babolin, V.E. Viana, A.C. de Oliveira, B. Gugi, E. Caporali, H. Herrera-Ubaldo, E. Martínez-Estrada, A. Driouich, S. de Folter, L. Colombo, I. Ezquer. - In: PLANTS. - ISSN 2223-7747. - 11:22(2022 Nov 17), pp. 3146.1-3146.23. [10.3390/plants11223146]

The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control

M. Di Marzo
Co-primo
;
N. Babolin
Co-primo
;
E. Caporali;L. Colombo
Penultimo
;
I. Ezquer
Ultimo
2022

Abstract

Although much is known about seed and fruit development at the molecular level, many gaps remain in our understanding of how cell wall modifications can impact developmental processes in plants, as well as how biomechanical alterations influence seed and fruit growth. Mutants of Arabidopsis thaliana constitute an excellent tool to study the function of gene families devoted to cell wall biogenesis. We have characterized a collection of lines carrying mutations in representative cell wall-related genes for seed and fruit size developmental defects, as well as altered germination rates. We have linked these studies to cell wall composition and structure. Interestingly, we have found that disruption of genes involved in pectin maturation and hemicellulose deposition strongly influence germination dynamics. Finally, we focused on two transcriptional regulators, SEEDSTICK (STK) and LEUNIG-HOMOLOG (LUH), which positively regulate seed growth. Herein, we demonstrate that these factors regulate specific aspects of cell wall properties such as pectin distribution. We propose a model wherein changes in seed coat structure due to alterations in the xyloglucan-cellulose matrix deposition and pectin maturation are critical for organ growth and germination. The results demonstrate the importance of cell wall properties and remodeling of polysaccharides as major factors responsible for seed development.
cell wall; transcriptional regulators; pectins; seed growth; fruit; germination
Settore BIO/01 - Botanica Generale
Settore BIO/18 - Genetica
Settore BIO/04 - Fisiologia Vegetale
JPI_MIPAAF21JEZQU_01 - CropsForChange; Tackling the global warming effects in crops (C4C) - EZQUER GARIN, JUAN IGNACIO - JPI_MIPAAF - Joint Programming Initiatives_MIPAAF - 2021
H20MC_RISE21LCOLO_01 - The polyploidy paradigm and its role in plant breeding (POLYPLOID) - COLOMBO, LUCIA - H20MC_RISE - Horizon 2020_Marie Skłodowska-Curie actions-International and inter-sectoral cooperation through the Research and Innovation Staff Exchanges (RISE) - 2021
PRIN201719LCOLO_01 - Evolution of molecular networks controlling seed coat differentiation - COLOMBO, LUCIA - PRIN2017 - PRIN bando 2017 - 2019
H20MC_RISE21LCOLO_02 - Evolution of genetic network required for fruit and fruit-like structures development of land plants (EVOfruland) - COLOMBO, LUCIA - H20MC_RISE - Horizon 2020_Marie Skłodowska-Curie actions-International and inter-sectoral cooperation through the Research and Innovation Staff Exchanges (RISE) - 2021
Article (author)
File in questo prodotto:
File Dimensione Formato  
plants-11-03146-v2.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 2.8 MB
Formato Adobe PDF
2.8 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/946268
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact