This study evaluates how long-term dietary low ω6:ω3 ratio in sows and offspring’s seaweed (SW) intake affects piglet intestinal function and growth through modifying ileum proteome. Sows were assigned to either control diet (CR, ω6:ω3 ratio = 13:1) or treatment diet (LR, ω6:ω3 = 4:1) during gestation and lactation (n = 8 each). The male weaned offspring were received a basal diet with or without SW powder Journal Pre-proof supplementation (4 g/kg) for 21 days, denoted as SW and CT groups, respectively. In total, four groups of weaned piglets were formed following maternal and offspring’s diets combination, represented by CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet ileum tissue was collected on day 22 post-weaning and analysed using TMT-based quantitative proteomics. The differentially abundant proteins (n = 300) showed the influence of maternal LR diet on protein synthesis, cell proliferation, and cell cycle regulation. In contrast, the SW diet lowered the inflammation severity and promoted ileal tissue development in CRSW piglets but reduced the fat absorption capacity in LRSW piglets. These results uncovered the mechanism behind the anti-inflammation and intestinal-boosting effects of maternal LR diet in piglets supplemented with SW.

Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II : Ileum proteomes / T.X. Nguyen, A. Agazzi, S. Mcgill, S. Weidt, Q.H. Han, A. Gelemanović, M. Mclaughlin, G. Savoini, P.D. Eckersall, R. Burchmore. - In: JOURNAL OF PROTEOMICS. - ISSN 1874-3919. - (2022 Sep 27). [Epub ahead of print] [10.1016/j.jprot.2022.104739]

Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II : Ileum proteomes

T.X. Nguyen
Primo
;
A. Agazzi
Secondo
;
G. Savoini;
2022

Abstract

This study evaluates how long-term dietary low ω6:ω3 ratio in sows and offspring’s seaweed (SW) intake affects piglet intestinal function and growth through modifying ileum proteome. Sows were assigned to either control diet (CR, ω6:ω3 ratio = 13:1) or treatment diet (LR, ω6:ω3 = 4:1) during gestation and lactation (n = 8 each). The male weaned offspring were received a basal diet with or without SW powder Journal Pre-proof supplementation (4 g/kg) for 21 days, denoted as SW and CT groups, respectively. In total, four groups of weaned piglets were formed following maternal and offspring’s diets combination, represented by CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet ileum tissue was collected on day 22 post-weaning and analysed using TMT-based quantitative proteomics. The differentially abundant proteins (n = 300) showed the influence of maternal LR diet on protein synthesis, cell proliferation, and cell cycle regulation. In contrast, the SW diet lowered the inflammation severity and promoted ileal tissue development in CRSW piglets but reduced the fat absorption capacity in LRSW piglets. These results uncovered the mechanism behind the anti-inflammation and intestinal-boosting effects of maternal LR diet in piglets supplemented with SW.
ileal proteome; ω6:ω3 fatty acids; seaweed; DNA and protein synthesis; weaned pigs; epithelial cells
Settore AGR/18 - Nutrizione e Alimentazione Animale
H20MCITNIF18FCECI_01 - Joint Doctorate in Molecular Animal Nutrition (MANNA) - CECILIANI, FABRIZIO - H20MCITNIF - Horizon 2020_Marie Skłodowska-Curie actions-Innovative Training Network (ITN)/Individual Fellowships (IF) - 2018
27-set-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/939821
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