By using the experimental design (DoE) technique, we optimized an analytical method for the determination of mercury isotope ratios by means of cold-vapor multicollector ICP-MS (CV-MC-ICP-MS) to provide absolute Hg isotopic ratio measurements with a suitable internal precision. By running 32 experiments, the influence of mercury and thallium internal standard concentrations, total measuring time and sample flow rate was evaluated. Method was optimized varying Hg concentration between 2 and 20 ng g-1. The model finds out some correlations within the parameters affect the measurements precision and predicts suitable sample measurement precisions for Hg concentrations from 5 ng g-1 Hg upwards. The method was successfully applied to samples of Manila clams (Ruditapes philippinarum) coming from the Marano and Grado lagoon (NE Italy), a coastal environment affected by long term mercury contamination mainly due to mining activity. Results show different extents of both mass dependent fractionation (MDF) and mass independent fractionation (MIF) phenomena in clams according to their size and sampling sites in the lagoon. The method is fit for determinations on real samples, allowing for the use of Hg isotopic ratios to study mercury biogeochemical cycles in complex ecosystems.

DoE optimization of a mercury isotope ratio determination method for environmental studies / A. Berni, C. Baschieri, S. Covelli, A. Emili, A. Marchetti, D. Manzini, D. Berto, F. Rampazzo. - In: TALANTA. - ISSN 0039-9140. - 152(2016 May 15), pp. 179-187. [10.1016/j.talanta.2016.02.012]

DoE optimization of a mercury isotope ratio determination method for environmental studies

C. Baschieri
Secondo
;
2016-05-15

Abstract

By using the experimental design (DoE) technique, we optimized an analytical method for the determination of mercury isotope ratios by means of cold-vapor multicollector ICP-MS (CV-MC-ICP-MS) to provide absolute Hg isotopic ratio measurements with a suitable internal precision. By running 32 experiments, the influence of mercury and thallium internal standard concentrations, total measuring time and sample flow rate was evaluated. Method was optimized varying Hg concentration between 2 and 20 ng g-1. The model finds out some correlations within the parameters affect the measurements precision and predicts suitable sample measurement precisions for Hg concentrations from 5 ng g-1 Hg upwards. The method was successfully applied to samples of Manila clams (Ruditapes philippinarum) coming from the Marano and Grado lagoon (NE Italy), a coastal environment affected by long term mercury contamination mainly due to mining activity. Results show different extents of both mass dependent fractionation (MDF) and mass independent fractionation (MIF) phenomena in clams according to their size and sampling sites in the lagoon. The method is fit for determinations on real samples, allowing for the use of Hg isotopic ratios to study mercury biogeochemical cycles in complex ecosystems.
DoE; MDF and MIF Hg fractionation processes; Mercury isotopes; Multi Linear Regression; Multicollector ICP-MS; Animals; Bivalvia; Environmental Monitoring; Environmental Pollutants; Humans; Mediterranean Sea; Mercury; Mercury Isotopes; Mining; Reference Standards; Spectrophotometry, Atomic; Thallium; Factor Analysis, Statistical
Settore CHIM/01 - Chimica Analitica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/928497
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