We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.
|Titolo:||Proteins of rat serum, urine, and cerebrospinal fluid : VI. Further protein identifications and interstrain comparison|
|Autori interni:||GIANAZZA, ELISABETTA (Secondo)|
|Parole Chiave:||rat ; serum ; urine ; cerebrospinal fluid ; two-dimensional electrophoresis|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||ago-2001|
|Digital Object Identifier (DOI):||10.1002/1522-2683(200108)22:14<3043::AID-ELPS3043>3.0.CO;2-M|
|Appare nelle tipologie:||01 - Articolo su periodico|