The chemical composition of Lebanese Hypericum scabrum essential oil (EO) was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GG-MS). Its antimicrobial activity was evaluated by determining its minimal inhibitory concentrations (MICs) against a Gram-negative and a Gram-positive bacterium, one yeast, and five dermatophytes. H. scabrum EO was most active on filamentous fungi (MIC values of 32-64 mu g/mL). Synergy within the oil was investigated by testing each of the following major components on Trichophyton rubrum: alpha-pinene, limonene, myrcene, beta-pinene and nonane, as well as a reconstructed EO. The antifungal activity of the natural oil could not be reached, meaning that its activity might be due, in part, to minor constituent(s). The interactions between H. scabrum EO and commercially available antifungals were assessed by the checkerboard test. A synergistic effect was revealed in the combination of the EO with amphotericin B.

Antimicrobial Activity and Synergy Investigation of Hypericum scabrum Essential Oil with Antifungal Drugs / L. Fahed, M. El Beyrouthy, N. Ouaini, V. Eparvier, D. Stien, S. Vitalini, M. Iriti. - In: MOLECULES. - ISSN 1420-3049. - 26:21(2021 Oct 29), pp. 6545.1-6545.8. [10.3390/molecules26216545]

Antimicrobial Activity and Synergy Investigation of Hypericum scabrum Essential Oil with Antifungal Drugs

S. Vitalini
Penultimo
;
M. Iriti
Ultimo
2021

Abstract

The chemical composition of Lebanese Hypericum scabrum essential oil (EO) was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GG-MS). Its antimicrobial activity was evaluated by determining its minimal inhibitory concentrations (MICs) against a Gram-negative and a Gram-positive bacterium, one yeast, and five dermatophytes. H. scabrum EO was most active on filamentous fungi (MIC values of 32-64 mu g/mL). Synergy within the oil was investigated by testing each of the following major components on Trichophyton rubrum: alpha-pinene, limonene, myrcene, beta-pinene and nonane, as well as a reconstructed EO. The antifungal activity of the natural oil could not be reached, meaning that its activity might be due, in part, to minor constituent(s). The interactions between H. scabrum EO and commercially available antifungals were assessed by the checkerboard test. A synergistic effect was revealed in the combination of the EO with amphotericin B.
Antimicrobial activity; Chemical composition; Essential oil; Hypericum scabrum; Synergy;
Settore BIO/01 - Botanica Generale
Settore AGR/12 - Patologia Vegetale
29-ott-2021
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/910787
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