Here we described the virological and serological assessment of 23 COVID-19 patients hospitalized and followed up in Milan, Italy, during the first wave of COVID-19 pandemic. Nasopharyngeal (NPS), anal swabs, and blood samples were collected from 23 COVID-19 patients, at hospital admission, and periodically up to discharge, for a median time of 20 days (3–83 days). RNA was isolated and tested for SARS-CoV-2 by qRT-PCR; anti-SARS-CoV-2 IgM and IgG antibody titers were evaluated in serum samples by ELISA. SARS-CoV-2 genome was detected in the NPS swabs of the 23 patients, at the admission, and 8/19 (42.1%) were still positive at the discharge. Anal swabs were positive to SARS-CoV-2 RNA detection in 20/23 (86.9%) patients; 6/19 (31.6%) were still positive at discharge. The mean time of RNA negative conversion was 17 days (4–36 days) and 33 days (4–77 days), for NPS and anal swabs, respectively. SARS-CoV-2-RNA was detected in the blood of 6/23 (26.1%) patients. Thirteen/23 (56.5%) and 17/23 (73.9%) patients were seropositive for IgM and IgG, respectively, at the admission, and the median IgM and IgG levels significantly (p < 0.05) increased after 13 days. Although the limited cohort size, our report provides evidence that SARS-CoV-2 is shed through multiple routes, with important implications in healthcare settings.

Longitudinal, virological, and serological assessment of hospitalized COVID-19 patients / L. Signorini, M. Dolci, N. Castelnuovo, L. Crespi, B. Incorvaia, P. Bagnoli, S. Parapini, N. Basilico, C. Galli, F. Ambrogi, E. Pariani, S. Binda, R. Ticozzi, P. Ferrante, S. Delbue. - In: JOURNAL OF NEUROVIROLOGY. - ISSN 1355-0284. - (2022). [Epub ahead of print] [10.1007/s13365-021-01029-0]

Longitudinal, virological, and serological assessment of hospitalized COVID-19 patients

Signorini L.;Dolci M.;Incorvaia B.;Parapini S.;Basilico N.;Galli C.;Ambrogi F.;Pariani E.;Binda S.;Ticozzi R.;Ferrante P.;Delbue S.
2022

Abstract

Here we described the virological and serological assessment of 23 COVID-19 patients hospitalized and followed up in Milan, Italy, during the first wave of COVID-19 pandemic. Nasopharyngeal (NPS), anal swabs, and blood samples were collected from 23 COVID-19 patients, at hospital admission, and periodically up to discharge, for a median time of 20 days (3–83 days). RNA was isolated and tested for SARS-CoV-2 by qRT-PCR; anti-SARS-CoV-2 IgM and IgG antibody titers were evaluated in serum samples by ELISA. SARS-CoV-2 genome was detected in the NPS swabs of the 23 patients, at the admission, and 8/19 (42.1%) were still positive at the discharge. Anal swabs were positive to SARS-CoV-2 RNA detection in 20/23 (86.9%) patients; 6/19 (31.6%) were still positive at discharge. The mean time of RNA negative conversion was 17 days (4–36 days) and 33 days (4–77 days), for NPS and anal swabs, respectively. SARS-CoV-2-RNA was detected in the blood of 6/23 (26.1%) patients. Thirteen/23 (56.5%) and 17/23 (73.9%) patients were seropositive for IgM and IgG, respectively, at the admission, and the median IgM and IgG levels significantly (p < 0.05) increased after 13 days. Although the limited cohort size, our report provides evidence that SARS-CoV-2 is shed through multiple routes, with important implications in healthcare settings.
Anal swabs; Anti-SARS-CoV-2 antibodies; Blood; Nasopharyngeal swabs; SARS-CoV-2
Settore MED/07 - Microbiologia e Microbiologia Clinica
7-gen-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/903185
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