Truncated antenna size of photosystems and lower leaf chlorophyll content has been shown to increase photosynthetic efficiency and biomass accumulation in microalgae, cyanobacteria and higher plants grown under high-density cultivation conditions. Here, we have asked whether this strategy is also applicable to a major crop by characterising the barley mutant happy under the sun 1 (hus1). The pale green phenotype of hus1 is due to a 50% reduction in the chlorophyll content of leaves, owing to a premature stop codon in the HvcpSRP43 gene for the 43-kDa chloroplast Signal Recognition Particle (cpSRP43). The HvcpSRP43 protein is responsible for the uploading of photosystem antenna proteins into the thylakoid membranes, and its truncation results in a smaller photosystem antenna size. Besides a detailed molecular and physiological characterization of the mutant grown under controlled greenhouse conditions, we show that the agronomic performance of hus1 plants, in terms of total biomass production and grain yield under standard field conditions, is comparable to that of control plants. The results are discussed in terms of the potential benefits of the hus1 phenotype, and of natural allelic variants of the HvcpSRP43 locus, with respect to productivity and mitigation of climate change.

The barley mutant happy under the sun 1 (hus1) : an additional contribution to pale green crops / L. Rotasperti, L. Tadini, M. Chiara, C. Crosatti, D. Guerra, A. Tagliani, S. Forlani, I. Ezquer, D.S. Horner, P. Jahns, K. Gajek, A. García, R. Savin, L. Rossini, A. Tondelli, A. Janiak, P. Pesaresi. - In: ENVIRONMENTAL AND EXPERIMENTAL BOTANY. - ISSN 0098-8472. - 196:(2022 Apr), pp. 104795.1-104795.21. [10.1016/j.envexpbot.2022.104795]

The barley mutant happy under the sun 1 (hus1) : an additional contribution to pale green crops

L. Rotasperti
Co-primo
;
L. Tadini
Co-primo
;
M. Chiara
Secondo
;
A. Tagliani;S. Forlani;I. Ezquer;D.S. Horner;L. Rossini;P. Pesaresi
Ultimo
Project Administration
2022

Abstract

Truncated antenna size of photosystems and lower leaf chlorophyll content has been shown to increase photosynthetic efficiency and biomass accumulation in microalgae, cyanobacteria and higher plants grown under high-density cultivation conditions. Here, we have asked whether this strategy is also applicable to a major crop by characterising the barley mutant happy under the sun 1 (hus1). The pale green phenotype of hus1 is due to a 50% reduction in the chlorophyll content of leaves, owing to a premature stop codon in the HvcpSRP43 gene for the 43-kDa chloroplast Signal Recognition Particle (cpSRP43). The HvcpSRP43 protein is responsible for the uploading of photosystem antenna proteins into the thylakoid membranes, and its truncation results in a smaller photosystem antenna size. Besides a detailed molecular and physiological characterization of the mutant grown under controlled greenhouse conditions, we show that the agronomic performance of hus1 plants, in terms of total biomass production and grain yield under standard field conditions, is comparable to that of control plants. The results are discussed in terms of the potential benefits of the hus1 phenotype, and of natural allelic variants of the HvcpSRP43 locus, with respect to productivity and mitigation of climate change.
photosystem antenna size; pale green leaves; photosynthesis efficiency; Hordeum vulgare; Arabidopsis thaliana
Settore BIO/18 - Genetica
Settore BIO/04 - Fisiologia Vegetale
   BarPLUS: modifying canopy architecture and photosynthesis to maximize barley biomass and yield for different end-uses
   BarPLUS
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
apr-2022
24-gen-2022
Article (author)
File in questo prodotto:
File Dimensione Formato  
Rotasperti et al_2022.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 929.49 kB
Formato Adobe PDF
929.49 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Manuscript_text.pdf

accesso aperto

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 2.12 MB
Formato Adobe PDF
2.12 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/900624
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 3
social impact