Stomata are epidermal pores formed by pairs of specialized guard cells, which regulate gas exchanges between the plant and the atmosphere. Modulation of transcription has emerged as an important level of regulation of stomatal activity. The AtMYB60 transcription factor was previously identified as a positive regulator of stomatal opening, although the details of its function remain unknown. Here, we propose a role for AtMYB60 as a negative modulator of oxylipins synthesis in stomata. The atmyb60-1 mutant shows reduced stomatal opening and accumulates increased levels of 12-oxo-phytodienoic acid (12-OPDA), jasmonic acid (JA) and jasmonoyl-l-isoleucine (JA-Ile) in guard cells. We provide evidence that 12-OPDA triggers stomatal closure independently of JA and cooperatively with abscisic acid (ABA) in atmyb60-1. Our study highlights the relevance of oxylipins metabolism in stomatal regulation and indicates AtMYB60 as transcriptional integrator of ABA and oxylipins responses in guard cells.

The AtMYB60 transcription factor regulates stomatal opening by modulating oxylipin synthesis in guard cells / F. Simeoni, A. Skirycz, L. Simoni, G. Castorina, L.P. de Souza, A.R. Fernie, S. Alseekh, P. Giavalisco, L. Conti, C. Tonelli, M. Galbiati. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 12:1(2022 Dec), pp. 533.1-533.12. [10.1038/s41598-021-04433-y]

The AtMYB60 transcription factor regulates stomatal opening by modulating oxylipin synthesis in guard cells

F. Simeoni
Primo
;
L. Simoni;G. Castorina;L. Conti;C. Tonelli
Penultimo
;
M. Galbiati
Ultimo
2022-12

Abstract

Stomata are epidermal pores formed by pairs of specialized guard cells, which regulate gas exchanges between the plant and the atmosphere. Modulation of transcription has emerged as an important level of regulation of stomatal activity. The AtMYB60 transcription factor was previously identified as a positive regulator of stomatal opening, although the details of its function remain unknown. Here, we propose a role for AtMYB60 as a negative modulator of oxylipins synthesis in stomata. The atmyb60-1 mutant shows reduced stomatal opening and accumulates increased levels of 12-oxo-phytodienoic acid (12-OPDA), jasmonic acid (JA) and jasmonoyl-l-isoleucine (JA-Ile) in guard cells. We provide evidence that 12-OPDA triggers stomatal closure independently of JA and cooperatively with abscisic acid (ABA) in atmyb60-1. Our study highlights the relevance of oxylipins metabolism in stomatal regulation and indicates AtMYB60 as transcriptional integrator of ABA and oxylipins responses in guard cells.
Settore BIO/18 - Genetica
11-gen-2022
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/895229
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