The thesis concerns the use of different enzyme classes (namely, oxidoreductases, transferases, and hydrolases) as biocatalysts to achieve the chemo-, regio- and stereo-selective green synthesis of pharmaceutically relevant compounds. The main work has been done with hydroxysteroid dehydrogenases, redox enzymes that naturally work on steroids, which were studied with two main purposes: to shorten the synthesis of valuable bile acids already sold as drugs or with potential biomarker application, and to synthesize active pharmaceutical ingredients of non-steroidal nature. The work has been completed by the study on a peculiar, extremely resistant, redox enzyme, which was isolated from a metagenome in search for novel thermostable hydroxyxsteroid dehydrogenases.

EXPLOITING OXIDOREDUCTASES, HYDROLASES AND TRANSAMINASES AS STEREO- AND REGIO-SELECTIVE BIOCATALYSTS IN ORGANIC SYNTHESIS / S. Bertuletti ; tutor: S. Riva; co-tutor: M. De Amici Prof. Giancarlo Aldini. - : . Dipartimento di Scienze Farmaceutiche, 2022 Jan 13. ((34. ciclo, Anno Accademico 2021.

EXPLOITING OXIDOREDUCTASES, HYDROLASES AND TRANSAMINASES AS STEREO- AND REGIO-SELECTIVE BIOCATALYSTS IN ORGANIC SYNTHESIS.

S. Bertuletti
2022

Abstract

The thesis concerns the use of different enzyme classes (namely, oxidoreductases, transferases, and hydrolases) as biocatalysts to achieve the chemo-, regio- and stereo-selective green synthesis of pharmaceutically relevant compounds. The main work has been done with hydroxysteroid dehydrogenases, redox enzymes that naturally work on steroids, which were studied with two main purposes: to shorten the synthesis of valuable bile acids already sold as drugs or with potential biomarker application, and to synthesize active pharmaceutical ingredients of non-steroidal nature. The work has been completed by the study on a peculiar, extremely resistant, redox enzyme, which was isolated from a metagenome in search for novel thermostable hydroxyxsteroid dehydrogenases.
RIVA, SERGIO
biocatalysis; HSDH; bile acid; substrate promiscuity; API
Settore CHIM/06 - Chimica Organica
Settore CHIM/08 - Chimica Farmaceutica
EXPLOITING OXIDOREDUCTASES, HYDROLASES AND TRANSAMINASES AS STEREO- AND REGIO-SELECTIVE BIOCATALYSTS IN ORGANIC SYNTHESIS / S. Bertuletti ; tutor: S. Riva; co-tutor: M. De Amici Prof. Giancarlo Aldini. - : . Dipartimento di Scienze Farmaceutiche, 2022 Jan 13. ((34. ciclo, Anno Accademico 2021.
Doctoral Thesis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/886073
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