Gelfoam® histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam® histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam® histoculture at the liquid-air interface. The Gelfoam® histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.

Histoculture and infection with HIV of functional human lymphoid tissue on Gelfoam® / A. Introini, W. Fitzgerald, C. Vanpouille, L. Margolis (METHODS IN MOLECULAR BIOLOGY). - In: 3D Sponge-Matrix Histoculture: Methods and Protocols / [a cura di] R.M. Hoffman. - [s.l] : Humana Press Inc., 2018. - ISBN 978-1-4939-7743-7. - pp. 187-197 [10.1007/978-1-4939-7745-1_17]

Histoculture and infection with HIV of functional human lymphoid tissue on Gelfoam®

A. Introini
Primo
;
2018

Abstract

Gelfoam® histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam® histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam® histoculture at the liquid-air interface. The Gelfoam® histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.
Antibody production; Gelfoam® ; Histoculture; HIV-1 infection; Lymphoid tissue; Tonsils
Settore MED/04 - Patologia Generale
Settore MED/50 - Scienze Tecniche Mediche Applicate
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/871609
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