Seed development is under the control of complex and coordinated molecular networks required for the formation of its different components. The seed coat development largely determines final seed size and shape, in addition to playing a crucial role in protecting the embryo and promoting germination. In this study, we investigated the role of three transcription factors known to be active during seed development in Arabidopsis thaliana: SEEDSTICK (STK) and GORDITA (GOA), two MADS-domain proteins, and AUXIN RESPONSE FACTOR 2 (ARF2), belonging to the ARF family. Through a reverse genetic approach, we characterized the seed phenotypes of all the single, double and triple loss-of-function mutants in relation to seed size/shape and the effects on metabolic pathways occurring in the seed coat. This approach revealed that dynamic networks involving these TFs are active throughout ovule and seed development, affecting the formation of the seed coat. Notably, while the genetic interaction among these genes results in synergies that control the promotion of cell expansion in the seed coat upon pollination and production of proanthocyanidins, functional antagonists arise in the control of cell proliferation and release of mucilage.
Genetic Interaction of SEEDSTICK, GORDITA and AUXIN RESPONSE FACTOR 2 during seed development / D. Paolo, G. Orozco Arroyo, L. Rotasperti, S. Masiero, L. Colombo, S. de Folter, B. Ambrose, E. Caporali, E. Ignacio, C. Mizzotti. - In: GENES. - ISSN 2073-4425. - 12:8(2021 Jul 30), pp. 1189.1-1189.12. [10.3390/genes12081189]
Titolo: | Genetic Interaction of SEEDSTICK, GORDITA and AUXIN RESPONSE FACTOR 2 during seed development | |
Autori: | MIZZOTTI, CHIARA (Corresponding) | |
Parole Chiave: | ARF; MADS; maternal tissues; seed development; transcription factors | |
Settore Scientifico Disciplinare: | Settore BIO/01 - Botanica Generale Settore BIO/18 - Genetica Settore BIO/04 - Fisiologia Vegetale | |
Progetto: | PRIN201719LCOLO_01 - Evolution of molecular networks controlling seed coat differentiation - COLOMBO, LUCIA - PRIN2017 - PRIN bando 2017 - 2019 7PQ_MC_IR14SMASI_M - The physiology and genetics of fruit formation: from genes to networks - MASIERO, SIMONA - 7PQ_MC_IR - 7 Programma Quadro_Marie Curie IRSES - 2014 2015-FDA-0010 - PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 3 "FINANZIAMENTO PER ACQUISTO/RINNOVO ATTREZZATURE SCIENTIFICHE FINALIZZATE ALLA RICERCA" - COLOMBO, LUCIA - PSR2015-17 - Piano di sviluppo di ricerca 2015-17 - 2015 PSR2015-1715LCOLO_M - PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE" - COLOMBO, LUCIA - PSR2015-17 - Piano di sviluppo di ricerca 2015-17 - 2015 | |
Data di pubblicazione: | 30-lug-2021 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.3390/genes12081189 | |
Appare nelle tipologie: | 01 - Articolo su periodico |
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