MicroRNAs are gene expression regulators associated with several human pathologies, including those generated by viral infections. Their role in SARS-CoV-2 infection and COVID-19 has been investigated and reviewed in many informative studies; however, a thorough miRNA outline in SARS-CoV-2-infected pregnant women (SIPW), at both systemic and placental levels, is missing. To fill this gap, blood and placenta biopsies collected at delivery from 15 asymptomatic SIPW were immediately analysed for: miRNA expression (n = 84) (QPCR array), antiviral/immune mRNA target expression (n = 74) (QGene) and cytokine/chemokines production (n = 27) (Multiplex ELISA). By comparing these results with those obtained from six uninfected pregnant women (UPW), we observed that, following SARS-CoV-2 infection, the transcriptomic profile of pregnant women is significantly altered in different anatomical districts, even in the absence of clinical symptoms and vertical transmission. This characteristic combination of miRNA and antiviral/immune factors seems to control both the infection and the dysfunctional immune reaction, thus representing a positive correlate of protection and a potential therapeutic target against SARS-CoV-2.

MiRNA profiling in plasma and placenta of SARS-CoV-2-infected pregnant women / I. Saulle, M. Garziano, C. Fenizia, G. Cappelletti, F. Parisi, M. Clerici, I. Cetin, V. Savasi, M. Biasin. - In: CELLS. - ISSN 2073-4409. - 10:7(2021 Jul 15), pp. 1788.1-1788.13. [10.3390/cells10071788]

MiRNA profiling in plasma and placenta of SARS-CoV-2-infected pregnant women

I. Saulle
Primo
;
M. Garziano;C. Fenizia;G. Cappelletti;M. Clerici;I. Cetin;V. Savasi;M. Biasin
Ultimo
2021

Abstract

MicroRNAs are gene expression regulators associated with several human pathologies, including those generated by viral infections. Their role in SARS-CoV-2 infection and COVID-19 has been investigated and reviewed in many informative studies; however, a thorough miRNA outline in SARS-CoV-2-infected pregnant women (SIPW), at both systemic and placental levels, is missing. To fill this gap, blood and placenta biopsies collected at delivery from 15 asymptomatic SIPW were immediately analysed for: miRNA expression (n = 84) (QPCR array), antiviral/immune mRNA target expression (n = 74) (QGene) and cytokine/chemokines production (n = 27) (Multiplex ELISA). By comparing these results with those obtained from six uninfected pregnant women (UPW), we observed that, following SARS-CoV-2 infection, the transcriptomic profile of pregnant women is significantly altered in different anatomical districts, even in the absence of clinical symptoms and vertical transmission. This characteristic combination of miRNA and antiviral/immune factors seems to control both the infection and the dysfunctional immune reaction, thus representing a positive correlate of protection and a potential therapeutic target against SARS-CoV-2.
SARS-CoV-2; pregnant women; MicroRNA; immune response
Settore BIO/13 - Biologia Applicata
Settore MED/04 - Patologia Generale
CAR_EXT20MBIAS_01 - Characterization of SARS-CoV-2 accessory proteins in terms of host adaptation, biochemical properties, and pharmacophore models (CORONA) - BIASIN, MARA - CAR_EXT - CARIPLO - EXTRABANDO E PROGETTI TERRITORIALI - 2020
RL_DG-WEL20MBIAS_01 - Caratterizzazione delle sequenze codificanti di SARS-CoV-2 e valutazione del loro ruolo nella patogenesi di COVID-19 - BIASIN, MARA - RL_DG-WEL - Bandi DG Welfare - 2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/858654
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