Despite substantial advancements have been achieved in the identification of long noncoding RNA (lncRNA) molecules, many challenges still remain into their functional characterization. Loss-of-function approaches are needed to study oncogenic lncRNAs, which appear more difficult to knock down by RNA interference as compared to mRNAs. In this chapter, we present a protocol based on the use of a novel class of antisense oligonucleotides, named locked nucleic acid (LNA) GapmeRs, to inhibit the oncogenic lncRNA NEAT1 in multiple myeloma cells. Overall, this approach holds many advantages, including its possible independence from delivery reagents as well as the capability to knock down lncRNAs even in hard-to-transfect suspension cells, like hematopoietic cells.

In Vitro Silencing of lncRNAs Using LNA GapmeRs / E. Taiana, V. Favasuli, D. Ronchetti, E. Morelli, P. Tassone, G. Viglietto, N.C. Munshi, A. Neri, N. Amodio (METHODS IN MOLECULAR BIOLOGY). - In: Long Non-Coding RNAs in Cancer[s.l] : Springer, 2021. - ISBN 9781071615805. - pp. 157-166 [10.1007/978-1-0716-1581-2_10]

In Vitro Silencing of lncRNAs Using LNA GapmeRs

E. Taiana;V. Favasuli;D. Ronchetti;A. Neri;
2021

Abstract

Despite substantial advancements have been achieved in the identification of long noncoding RNA (lncRNA) molecules, many challenges still remain into their functional characterization. Loss-of-function approaches are needed to study oncogenic lncRNAs, which appear more difficult to knock down by RNA interference as compared to mRNAs. In this chapter, we present a protocol based on the use of a novel class of antisense oligonucleotides, named locked nucleic acid (LNA) GapmeRs, to inhibit the oncogenic lncRNA NEAT1 in multiple myeloma cells. Overall, this approach holds many advantages, including its possible independence from delivery reagents as well as the capability to knock down lncRNAs even in hard-to-transfect suspension cells, like hematopoietic cells.
No
English
ncRNA; lncRNA; ASO; LNA-GapmeR; Electroporation; Gymnosis; Multiple myeloma cells
Settore BIO/11 - Biologia Molecolare
Capitolo o Saggio
Esperti anonimi
Pubblicazione scientifica
Long Non-Coding RNAs in Cancer
Springer
2021
157
166
10
9781071615805
9781071615812
2348
Volume a diffusione internazionale
pubmed
crossref
Aderisco
E. Taiana, V. Favasuli, D. Ronchetti, E. Morelli, P. Tassone, G. Viglietto, N.C. Munshi, A. Neri, N. Amodio
Book Part (author)
reserved
268
In Vitro Silencing of lncRNAs Using LNA GapmeRs / E. Taiana, V. Favasuli, D. Ronchetti, E. Morelli, P. Tassone, G. Viglietto, N.C. Munshi, A. Neri, N. Amodio (METHODS IN MOLECULAR BIOLOGY). - In: Long Non-Coding RNAs in Cancer[s.l] : Springer, 2021. - ISBN 9781071615805. - pp. 157-166 [10.1007/978-1-0716-1581-2_10]
info:eu-repo/semantics/bookPart
9
Prodotti della ricerca::03 - Contributo in volume
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/853840
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