Background and aims. In recent years, there is growing evidence that plant-foods polyphenols, due to their biological properties, may be unique nutraceuticals and supplementary treatments for various aspects of type 2 diabetes mellitus. Verbascoside/Acteoside (VB), a phenylethanoid glycoside (PhGs), is a widespread polyphenolic plant compound with several biological properties, including strong antioxidant and neuroprotective activities. Whether it is active also on islets of Langerhans is unknown. Aim of the proposed research was to verify the protective effects of VB on oxidative stress in beta cells and to verify the molecular mechanisms of this modulation. Materials and methods. Mouse βTC3 cells were incubated with different VB concentrations (for up to 5 days) and the beta cell survival was evaluated with MTT test and flow cytometry in normal growing conditions or after exposure to H2O2 (0,5 mM) for 20 minutes. Reactive oxidative species (ROS) were detected with fluorescence assays and mitochondrial activity and dynamics were evaluated by fluorescence microscopy. The expression of antioxidant enzymes, ER stress and inflammatory markers were analysed by western blotting. Results. We found that chronic exposure of βTC3 cell lines to VB (1-100 μM) improved beta cell survival and significantly prevented beta cell death induced by oxidative stress. We investigated the mechanism of this protection and we found that the increased β cell survival in VB treated cells was associated with improved mitochondrial functions and dynamics, decreased ROS production (20% reduction, p<0,05; n=3) and lipid oxidation (50% reduction acrolein, p<0,05; n=4). Interestingly, western blotting analysis showed reduced expression of ER stress markers and downregulation of NF-κB signalling pathway in β-cells treated with VB. Conclusions. Our results indicate that VB enhances beta cell function via modulation of ROS production and attenuation of NF-κB signaling pathway. The study provides evidence that administration of VB may be helpful in preventing oxidative stress and inflammation in islet of Langerhans exposed to oxidative stress.

Verbascoside, a phenylethanoid glycoside, controls pancreatic beta-cell survival and function by decreasing oxidative stress and inflammation / A. Galli, C. Perego. ((Intervento presentato al 8. convegno Next step : La giovane ricerca avanza tenutosi a Milano nel 2017.

Verbascoside, a phenylethanoid glycoside, controls pancreatic beta-cell survival and function by decreasing oxidative stress and inflammation

A. Galli;C. Perego
2017-06

Abstract

Background and aims. In recent years, there is growing evidence that plant-foods polyphenols, due to their biological properties, may be unique nutraceuticals and supplementary treatments for various aspects of type 2 diabetes mellitus. Verbascoside/Acteoside (VB), a phenylethanoid glycoside (PhGs), is a widespread polyphenolic plant compound with several biological properties, including strong antioxidant and neuroprotective activities. Whether it is active also on islets of Langerhans is unknown. Aim of the proposed research was to verify the protective effects of VB on oxidative stress in beta cells and to verify the molecular mechanisms of this modulation. Materials and methods. Mouse βTC3 cells were incubated with different VB concentrations (for up to 5 days) and the beta cell survival was evaluated with MTT test and flow cytometry in normal growing conditions or after exposure to H2O2 (0,5 mM) for 20 minutes. Reactive oxidative species (ROS) were detected with fluorescence assays and mitochondrial activity and dynamics were evaluated by fluorescence microscopy. The expression of antioxidant enzymes, ER stress and inflammatory markers were analysed by western blotting. Results. We found that chronic exposure of βTC3 cell lines to VB (1-100 μM) improved beta cell survival and significantly prevented beta cell death induced by oxidative stress. We investigated the mechanism of this protection and we found that the increased β cell survival in VB treated cells was associated with improved mitochondrial functions and dynamics, decreased ROS production (20% reduction, p<0,05; n=3) and lipid oxidation (50% reduction acrolein, p<0,05; n=4). Interestingly, western blotting analysis showed reduced expression of ER stress markers and downregulation of NF-κB signalling pathway in β-cells treated with VB. Conclusions. Our results indicate that VB enhances beta cell function via modulation of ROS production and attenuation of NF-κB signaling pathway. The study provides evidence that administration of VB may be helpful in preventing oxidative stress and inflammation in islet of Langerhans exposed to oxidative stress.
Settore BIO/09 - Fisiologia
Università degli Studi di Milano-Dipartimento di Scienze Farmacologiche e Biomolecolari (DiSFeB)
Verbascoside, a phenylethanoid glycoside, controls pancreatic beta-cell survival and function by decreasing oxidative stress and inflammation / A. Galli, C. Perego. ((Intervento presentato al 8. convegno Next step : La giovane ricerca avanza tenutosi a Milano nel 2017.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/853626
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