Maternal obesity and gestational diabetes mellitus (GDM) are increasing worldwide, repre-senting risk factors for both mother and child short/long-term outcomes. Oxidative stress, lipotoxicity and altered autophagy have already been reported in obesity, but few studies have focused on obese pregnant women with GDM. Antioxidant and macro/chaperone-mediated autophagy (CMA)-related gene expressions were evaluated herein in obese and GDM placentas. A total of 47 women with singleton pregnancies delivered by elective cesarean section were enrolled: 16 normal weight (NW), 18 obese with no comorbidities (OB GDM(–)), 13 obese with GDM (OB GDM(+)). Placental gene expression was assessed by real-time PCR. Antioxidant gene expression (CAT, GPX1, GSS) decreased, the pro-autophagic ULK1 gene increased and the chaperone-mediated autophagy regulator PHLPP1 decreased in OB GDM(–) vs. NW. On the other hand, PHLPP1 expression increased in OB GDM(+) vs. OB GDM(–). When analyzing results in relation to fetal sex, we found sexual dimorphism for both antioxidant and CMA-related gene expressions. These preliminary results can pave the way for further analyses aimed at elucidating the placental autophagy role in metabolic pregnancy disorders and its potential targetability for the treatment of diabetes outcomes.

Placental antioxidant defenses and autophagy-related genes in maternal obesity and gestational diabetes mellitus / C. Diceglie, G.M. Anelli, C. Martelli, A. Serati, A. Lo Dico, F. Lisso, F. Parisi, C. Novielli, R. Paleari, I. Cetin, L. Ottobrini, C. Mando'. - In: NUTRIENTS. - ISSN 2072-6643. - 13:4(2021 Apr), pp. 1303.1-1303.18. [10.3390/nu13041303]

Placental antioxidant defenses and autophagy-related genes in maternal obesity and gestational diabetes mellitus

C. Diceglie
Co-primo
;
G.M. Anelli
Co-primo
;
C. Martelli;A. Serati;A. Lo Dico;F. Lisso;C. Novielli;R. Paleari;I. Cetin;L. Ottobrini
Penultimo
;
C. Mando'
Ultimo
2021

Abstract

Maternal obesity and gestational diabetes mellitus (GDM) are increasing worldwide, repre-senting risk factors for both mother and child short/long-term outcomes. Oxidative stress, lipotoxicity and altered autophagy have already been reported in obesity, but few studies have focused on obese pregnant women with GDM. Antioxidant and macro/chaperone-mediated autophagy (CMA)-related gene expressions were evaluated herein in obese and GDM placentas. A total of 47 women with singleton pregnancies delivered by elective cesarean section were enrolled: 16 normal weight (NW), 18 obese with no comorbidities (OB GDM(–)), 13 obese with GDM (OB GDM(+)). Placental gene expression was assessed by real-time PCR. Antioxidant gene expression (CAT, GPX1, GSS) decreased, the pro-autophagic ULK1 gene increased and the chaperone-mediated autophagy regulator PHLPP1 decreased in OB GDM(–) vs. NW. On the other hand, PHLPP1 expression increased in OB GDM(+) vs. OB GDM(–). When analyzing results in relation to fetal sex, we found sexual dimorphism for both antioxidant and CMA-related gene expressions. These preliminary results can pave the way for further analyses aimed at elucidating the placental autophagy role in metabolic pregnancy disorders and its potential targetability for the treatment of diabetes outcomes.
Antioxidant defenses; Autophagy; Chaperone-mediated autophagy (CMA); GDM; Macroautophagy; Maternal obesity; Placenta; Pregnancy; Sexual dimorphism;
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Settore MED/40 - Ginecologia e Ostetricia
Settore MED/50 - Scienze Tecniche Mediche Applicate
PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE"
PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE"
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/843948
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