Objectives: The analytical performance of a new automated HPLC system, for the determination of HbA1C in blood (Tosoh HLC-723 G7), was studied. Design and methods: The study design included the evaluation of imprecision, linearity, interference and carryover. Comparison study was performed by comparing HbA1C results with those obtained from an established method (Bio-Rad Variant II). Results: Total imprecision was less than 1.34% and the results were linear up to 17.2% HbA1C. The method showed a wide analytical range, and no carryover between specimens. Comparison study yielded, r = 0.989, Sy.x = 0.255, regression equation (y = 0.9895x-0.35); Bland-Altman plot showed a mean bias = - 0.43% HbA1C with confidence limits ranging from -0.48% to -0.38% HbA1C. The presence of abnormal hemoglobin was clearly revealed, and no interference from labile HbA 1C was apparent. Conclusion: The HLC-723 G7 instrument seems to be a reliable system for routine assay of HbA1C.

Evaluation of the analytic performances of the new HPLC system HLC-723 G7 for the measurement of hemoglobin A1c / A. Terreni, R. Paleari, A. Caldini, A. Ognibene, A. Mosca, G. Messeri. - In: CLINICAL BIOCHEMISTRY. - ISSN 0009-9120. - 36:8(2003 Nov), pp. 607-610.

Evaluation of the analytic performances of the new HPLC system HLC-723 G7 for the measurement of hemoglobin A1c

R. Paleari
Secondo
;
A. Mosca
Penultimo
;
2003

Abstract

Objectives: The analytical performance of a new automated HPLC system, for the determination of HbA1C in blood (Tosoh HLC-723 G7), was studied. Design and methods: The study design included the evaluation of imprecision, linearity, interference and carryover. Comparison study was performed by comparing HbA1C results with those obtained from an established method (Bio-Rad Variant II). Results: Total imprecision was less than 1.34% and the results were linear up to 17.2% HbA1C. The method showed a wide analytical range, and no carryover between specimens. Comparison study yielded, r = 0.989, Sy.x = 0.255, regression equation (y = 0.9895x-0.35); Bland-Altman plot showed a mean bias = - 0.43% HbA1C with confidence limits ranging from -0.48% to -0.38% HbA1C. The presence of abnormal hemoglobin was clearly revealed, and no interference from labile HbA 1C was apparent. Conclusion: The HLC-723 G7 instrument seems to be a reliable system for routine assay of HbA1C.
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/8411
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 19
social impact