The correlation between iron chlorosis resistance and induction of adaptive mechanisms in grapevine calli belonging to cultivars with different susceptibility to iron chlorosis has been investigated. Fe(III)-chelate reductase was clearly linked to the Fe-efficiency status of the genotype. When growing on iron deprived medium (-Fe) calli of the Fe-efficient genotype Cabernet sauvignon showed a remarkable increase in enzyme activity, up to five times higher, with respect to +Fe cultures. Moreover, P-31-NMR revealed that in -Fe medium the increase of vacuolar Pi content of the Fe-efficient cultures was more pronounced than that recorded for the Fe-inefficient Vitis riparia. Furthermore, Fe starvation also enhanced the production of phenolic compounds in calli of Cabernet sauvignon with respect to those of Vitis riparia. The role of H+-ATPase as a marker of Fe-efficiency in tissue culture remains ambiguous in the case of grapevines.
Adaptive responses to iron deficiency in callus cultures of two cultivars of Vitis spp. / C. Piagnani, P. De Nisi, L. Espen, G. Zocchi. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - 160:8(2003 Aug), pp. 865-870.
|Titolo:||Adaptive responses to iron deficiency in callus cultures of two cultivars of Vitis spp.|
PIAGNANI, MARIA CLAUDIA (Primo)
DE NISI, PATRIZIA (Secondo)
ESPEN, LUCA (Penultimo)
ZOCCHI, GRAZIANO (Ultimo)
|Parole Chiave:||Fe(III)-chelate reductase; H<sup>+</sup>-ATPase; Intracellular pH; Phenolic compounds; Vacuolar P<inf>j</inf>|
|Settore Scientifico Disciplinare:||Settore AGR/13 - Chimica Agraria|
Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree
|Data di pubblicazione:||ago-2003|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1078/0176-1617-00993|
|Appare nelle tipologie:||01 - Articolo su periodico|