The correlation between iron chlorosis resistance and induction of adaptive mechanisms in grapevine calli belonging to cultivars with different susceptibility to iron chlorosis has been investigated. Fe(III)-chelate reductase was clearly linked to the Fe-efficiency status of the genotype. When growing on iron deprived medium (-Fe) calli of the Fe-efficient genotype Cabernet sauvignon showed a remarkable increase in enzyme activity, up to five times higher, with respect to +Fe cultures. Moreover, P-31-NMR revealed that in -Fe medium the increase of vacuolar Pi content of the Fe-efficient cultures was more pronounced than that recorded for the Fe-inefficient Vitis riparia. Furthermore, Fe starvation also enhanced the production of phenolic compounds in calli of Cabernet sauvignon with respect to those of Vitis riparia. The role of H+-ATPase as a marker of Fe-efficiency in tissue culture remains ambiguous in the case of grapevines.

Adaptive responses to iron deficiency in callus cultures of two cultivars of Vitis spp. / C. Piagnani, P. De Nisi, L. Espen, G. Zocchi. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - 160:8(2003 Aug), pp. 865-870.

Adaptive responses to iron deficiency in callus cultures of two cultivars of Vitis spp.

C. Piagnani;P. De Nisi;L. Espen;G. Zocchi
2003-08

Abstract

The correlation between iron chlorosis resistance and induction of adaptive mechanisms in grapevine calli belonging to cultivars with different susceptibility to iron chlorosis has been investigated. Fe(III)-chelate reductase was clearly linked to the Fe-efficiency status of the genotype. When growing on iron deprived medium (-Fe) calli of the Fe-efficient genotype Cabernet sauvignon showed a remarkable increase in enzyme activity, up to five times higher, with respect to +Fe cultures. Moreover, P-31-NMR revealed that in -Fe medium the increase of vacuolar Pi content of the Fe-efficient cultures was more pronounced than that recorded for the Fe-inefficient Vitis riparia. Furthermore, Fe starvation also enhanced the production of phenolic compounds in calli of Cabernet sauvignon with respect to those of Vitis riparia. The role of H+-ATPase as a marker of Fe-efficiency in tissue culture remains ambiguous in the case of grapevines.
Fe(III)-chelate reductase; H+-ATPase; Intracellular pH; Phenolic compounds; Vacuolar Pj
Settore AGR/13 - Chimica Agraria
Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/8354
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