The formation of reversible-covalent interactions between a small-molecule ligand and its protein target is emerging as a general strategy to design binders with increased affinity. In this context, 2-hydroxybenzaldehyde (2HB) has been recently proposed as suitable electrophilic tag to engage primary amines, such as the ϵ-amino group of lysine residues, in remarkably stable imines. Lys residues are often expressed in high amounts on protein surfaces and in the proximity of ligand binding sites, and a fine-tuning of the chemical connection between the ligand and 2HB is fundamental for the affinity gain. Herein we report the synthesis of four 2HB tags functionalized with a short polyethylene glycol (PEG) spacer and a suitable reactive ‘handle’ (alkyne, azide, carboxylic acid and amine, respectively) for their conjugation to virtually any type of small molecule ligand.
Functionalized 2-Hydroxybenzaldehyde-PEG Modules as Portable Tags for the Engagement of Protein Lysine ϵ-Amino Groups / G. Sacco, S. Stammwitz, L. Belvisi, L. Pignataro, A. Dal Corso, C. Gennari. - In: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY. - ISSN 1434-193X. - 2021:11(2021), pp. 1763-1767.
|Titolo:||Functionalized 2-Hydroxybenzaldehyde-PEG Modules as Portable Tags for the Engagement of Protein Lysine ϵ-Amino Groups|
SACCO, GIOVANNI (Primo)
DAL CORSO, ALBERTO (Penultimo) (Corresponding)
GENNARI, CESARE MARIO ARTURO (Ultimo) (Corresponding)
|Parole Chiave:||Aldehydes; Conjugation; Regioselectivity; Schiff bases; Skattebø l formylation|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
|Data di pubblicazione:||2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/ejoc.202100160|
|Appare nelle tipologie:||01 - Articolo su periodico|