In cancer, myeloid cells have tumor-supporting roles. We reported that the protein GPNMB (glycoprotein nonmetastatic B) was profoundly upregulated in macrophages interacting with tumor cells. Here, using mouse tumor models, we show that macrophage-derived soluble GPNMB increases tumor growth and metastasis in Gpnmb-mutant mice (DBA/2J). GPNMB triggers in the cancer cells the formation of self-renewing spheroids, which are characterized by the expression of cancer stem cell markers, prolonged cell survival and increased tumor-forming ability. Through the CD44 receptor, GPNMB mechanistically activates tumor cells to express the cytokine IL-33 and its receptor IL-1R1L. We also determined that recombinant IL-33 binding to IL-1R1L is sufficient to induce tumor spheroid formation with features of cancer stem cells. Overall, our results reveal a new paracrine axis, GPNMB and IL-33, which is activated during the cross talk of macrophages with tumor cells and eventually promotes cancer cell survival, the expansion of cancer stem cells and the acquisition of a metastatic phenotype.
The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33 / M. Liguori, E. Digifico, A. Vacchini, R. Avigni, F.S. Colombo, E.M. Borroni, F.M. Farina, S. Milanesi, A. Castagna, L. Mannarino, I. Craparotta, S. Marchini, E. Erba, N. Panini, M. Tamborini, V. Rimoldi, P. Allavena, C. Belgiovine. - In: CELLULAR & MOLECULAR IMMUNOLOGY. - ISSN 1672-7681. - 18:3(2021 Mar), pp. 711-722.
|Titolo:||The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33|
LIGUORI, MANUELA (Primo)
|Parole Chiave:||cancer stem cells; GPNMB; IL-33; metastasis; tumor associated macrophages;|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||mar-2021|
|Data ahead of print / Data di stampa:||lug-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41423-020-0501-0|
|Appare nelle tipologie:||01 - Articolo su periodico|