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EnglishOvarian failure is the most common cause of infertility and affects about 1% of young women. One innovative strategy to restore ovarian function may be represented by the development of a bioprosthetic ovary, obtained through the combination of tissue engineering and regenerative medicine.We here describe the two main steps required for bioengineering the ovary and for its ex vivo functional reassembling. The first step aims at producing a 3D bioscaffold, which mimics the natural ovarian milieu in vitro. This is obtained with a whole organ decellularization technique that allows the maintenance of microarchitecture and biological signals of the original tissue. The second step involves the use of magnetic activated cell sorting (MACS) to isolate purified female germline stem cells (FGSCs). These cells are able to differentiate in ovarian adult mature cells, when subjected to specific stimuli, and can be used them to repopulate ovarian decellularized bioscaffolds. The combination of the two techniques represents a powerful tool for in vitro recreation of a bioengineered ovary that may constitute a promising solution for hormone and fertility function restoring. In addition, the procedures here described allow for the creation of a suitable 3D platform with useful applications both in toxicological and transplantation studies.
Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bioscaffold / G. Pennarossa, M. Ghiringhelli, F. Gandolfi, T.A. Brevini (METHODS IN MOLECULAR BIOLOGY). - In: Next Generation Culture Platforms for Reliable In Vitro Models / [a cura di] T.A.L. Brevini, A. Fazeli, K. Turksen. - [s.l] : Humana, 2021. - ISBN 9781071612453. - pp. 139-149
| Titolo: | Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bioscaffold | |
| Autori: | ||
| Parole Chiave: | 3D bioscaffold; Bioprosthetic ovary; Extracellular matrix; Female germline stem cells; Ovary bioengineering and repopulation; Whole-ovary decellularization | |
| Settore Scientifico Disciplinare: | Settore VET/01 - Anatomia degli Animali Domestici | |
| Data di pubblicazione: | 2021 | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/978-1-0716-1246-0_9 | |
| Tipologia: | Book Part (author) | |
| Appare nelle tipologie: | 03 - Contributo in volume |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Pennarossa et al_MiMB.pdf | Post-print, accepted manuscript ecc. (versione accettata dall'editore) | Open Access Visualizza/Apri | ||
| Pennarossa2021_Protocol_CreationOfABioengineeredOvaryI.pdf | Publisher's version/PDF | Administrator Richiedi una copia |
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