SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments and vaccines. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokines production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune booster of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro / C. Salaris, M. Scarpa, M. Elli, A. Bertolini, S. Guglielmetti, F. Pregliasco, C. Blandizzi, P. Brun, I. Castagliuolo. - In: NUTRIENTS. - ISSN 2072-6643. - 13:2(2021 Feb), pp. 328.1-328.12.
|Titolo:||Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro|
|Parole Chiave:||COVIS-19; lactoferrin|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
|Data di pubblicazione:||23-gen-2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/nu13020328|
|Appare nelle tipologie:||01 - Articolo su periodico|