Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.
Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension / M. Biancospino, G.R. Buel, C.A. Nino, E. Maspero, R.S. di Perrotolo, A. Raimondi, L. Redlingshofer, J. Weber, F.M. Brodsky, K.J. Walters, S. Polo. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 10:1(2019 Oct).
|Titolo:||Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension|
BIANCOSPINO, MATTEO (Primo)
POLO, SIMONA LAURA ANNA (Ultimo) (Corresponding)
|Parole Chiave:||Actins; Adaptor Proteins, Signal Transducing; Caco-2 Cells; Cell Culture Techniques; Clathrin Light Chains; Clathrin-Coated Vesicles; Coated Pits, Cell-Membrane; Cysts; Endocytosis; Humans; Magnetic Resonance Spectroscopy; Microfilament Proteins; Myosin Heavy Chains; Protein Binding; Protein Conformation; Protein Isoforms|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||ott-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41467-019-12855-6|
|Appare nelle tipologie:||01 - Articolo su periodico|