Phaeodactylum tricornutum was cultivated mixotrophically in batch mode providing glycerol as the C source, i.e., 0.02, 0.03 and 0.04 Mol L−1 glycerol, and ultrafiltered digestate (UF) as an N source. Biomass productivity, biomass composition, N efficiency use and total energy balance were recorded and compared to those under autotrophic conditions. Under mixotrophic conditions (0.03 Mol L-1 and 0.04 Mol L-1 glycerol), biomass productivity of P. tricornutum increased by 1.29 and 1.60 times in comparison with autotrophic conditions. Algal protein content declined as glycerol concentration increased, contrary to the case of the carbohydrate content. Lipid content did not change but unexpectedly, a lower unsaturated fatty acid in mixotrophic culture was observed than that from autotrophic culture. Mixotrophic conditions offered a higher energy recovery efficiency (EFt) than autotrophic conditions (5.7 % in 0.04 Mol L−1 glycerol and 4.2 % in autotrophic trial, respectively). Additionally, the efficiency of glycerol conversion into biomass (EFgly) increased with the glycerol dose, achieving 22.8 % for 0.04 Mol L−1 glycerol.

Phaeodactylum tricornutum cultivation under mixotrophic conditions with glycerol supplied with ultrafiltered digestate: A simple biorefinery approach recovering C and N / M. Su, G. D'Imporzano, D. Veronesi, S. Afric, F. Adani. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 323:(2020 Nov 10), pp. 73-81. [10.1016/j.jbiotec.2020.07.018]

Phaeodactylum tricornutum cultivation under mixotrophic conditions with glycerol supplied with ultrafiltered digestate: A simple biorefinery approach recovering C and N

M. Su;G. D'Imporzano
;
D. Veronesi;F. Adani
2020

Abstract

Phaeodactylum tricornutum was cultivated mixotrophically in batch mode providing glycerol as the C source, i.e., 0.02, 0.03 and 0.04 Mol L−1 glycerol, and ultrafiltered digestate (UF) as an N source. Biomass productivity, biomass composition, N efficiency use and total energy balance were recorded and compared to those under autotrophic conditions. Under mixotrophic conditions (0.03 Mol L-1 and 0.04 Mol L-1 glycerol), biomass productivity of P. tricornutum increased by 1.29 and 1.60 times in comparison with autotrophic conditions. Algal protein content declined as glycerol concentration increased, contrary to the case of the carbohydrate content. Lipid content did not change but unexpectedly, a lower unsaturated fatty acid in mixotrophic culture was observed than that from autotrophic culture. Mixotrophic conditions offered a higher energy recovery efficiency (EFt) than autotrophic conditions (5.7 % in 0.04 Mol L−1 glycerol and 4.2 % in autotrophic trial, respectively). Additionally, the efficiency of glycerol conversion into biomass (EFgly) increased with the glycerol dose, achieving 22.8 % for 0.04 Mol L−1 glycerol.
Energy balance; Glycerol; Mixotrophy; N recovery; Phaeodactylum tricornutum
Settore AGR/13 - Chimica Agraria
10-nov-2020
Article (author)
File in questo prodotto:
File Dimensione Formato  
Su Min -PDF.pdf

accesso riservato

Tipologia: Altro
Dimensione 402.66 kB
Formato Adobe PDF
402.66 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
1-s2.0-S0168165620302005-main.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 666.06 kB
Formato Adobe PDF
666.06 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/787093
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact