Histology, immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR) have been used to diagnose feline infectious peritonitis (FIP), but no information regarding the comparison of their diagnostic performances on the same organ is available. The aims of this study were to determine the concordance among these tests and to evaluate which combination of tests and organs can be used in vivo. Histology, IHC, and nested RT-PCR (RT-nPCR) for feline coronavirus (FCoV) were performed on spleen, liver, mesenteric lymph node, kidney, large and small intestine, and lung from 14 FIP and 12 non-FIP cats. Sensitivity, specificity, predictive values, likelihood ratios, and concordance were calculated. IHC and RT-nPCR had the highest concordance in lung and liver, histology and IHC in the other organs. The sensitivity of histology, IHC, and RT-nPCR on the different organs ranged from 41.7 to 76.9%, 46.2 to 76.9%, and 64.3 to 85.7%, respectively, and their specificity ranged from 83.3 to 100.0%, 100% and 83.3 to 100.0%. Therefore, IHC is recommended when histology is consistent with FIP. If RT-nPCR is performed as the first diagnostic approach, results should always be confirmed with IHC. Lung or liver provide accurate information regardless of the method, while IHC is preferred to RT-nPCR to confirm FIP in the kidney or intestine.
Concordance between histology, immunohistochemistry, and Rt-Pcr in the diagnosis of feline infectious peritonitis / A. Stranieri, D. Scavone, S. Paltrinieri, A. Giordano, F. Bonsembiante, S. Ferro, M.E. Gelain, S. Meazzi, S. Lauzi. - In: PATHOGENS. - ISSN 2076-0817. - 9:10(2020 Oct 18), pp. 852.1-852.15.
|Titolo:||Concordance between histology, immunohistochemistry, and Rt-Pcr in the diagnosis of feline infectious peritonitis|
STRANIERI, ANGELICA (Primo)
SCAVONE, DONATELLA (Secondo)
PALTRINIERI, SAVERIO (Corresponding)
MEAZZI, SARA (Penultimo)
LAUZI, STEFANIA (Ultimo)
|Parole Chiave:||Agreement; Cat; Diagnosis; Feline coronavirus; Feline infectious peritonitis; FIP; Immunohistochemistry; RT-PCR|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
|Data di pubblicazione:||18-ott-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/pathogens9100852|
|Appare nelle tipologie:||01 - Articolo su periodico|