In angiosperms, floral homeotic genes encoding MADS-domain transcription factors regulate the development of floral organs. Specifically, members of the SEPALLATA (SEP) and AGAMOUS (AG) subfamilies form higher order protein complexes to control Floral Meristem determinacy and to specify the identity of female reproductive organs. In rice, the AG subfamily gene OsMADS13 is intimately involved in the determination of ovule identity, since knock-out mutant plants develop carpel-like structures in place of ovules resulting in female sterility. Little is known about the regulatory pathways at the base of rice gynoecium development. To further investigate molecular mechanisms acting downstream of OsMADS13, we obtained transcriptomes of immature inflorescences from wild type and the osmads13 mutant. Among a total of 476 Differentially Expressed Genes (DEGs), a substantial overlap with DEGs from the SEP-family osmads1 mutant was found, suggesting that OsMADS1 and OsMADS13 may act on a common set of target genes. Expression studies and preliminary analyses of two upregulated Zinc-finger transcription factor encoding genes indicate that our dataset represents a valuable resource for the identification of both OsMADS13 target genes and novel players in rice ovule development. Taken together, our study suggests that OsMADS13 is an important repressor of the carpel pathway during ovule development.

Transcriptome analysis reveals rice OsMADS13 as an important repressor of the carpel development pathway in ovules / M. Osnato, E. Lacchini, A. Pilatone, L. Dreni, A. Grioni, M. Chiara, D. Horner, S. Pelaz, M.M. Kater. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - (2020). [Epub ahead of print] [10.1093/jxb/eraa460]

Transcriptome analysis reveals rice OsMADS13 as an important repressor of the carpel development pathway in ovules

Osnato, Michela;Lacchini, Elia;Pilatone, Alessandro;Dreni, Ludovico;Chiara, Matteo;Horner, David;Kater, Martin M
2020

Abstract

In angiosperms, floral homeotic genes encoding MADS-domain transcription factors regulate the development of floral organs. Specifically, members of the SEPALLATA (SEP) and AGAMOUS (AG) subfamilies form higher order protein complexes to control Floral Meristem determinacy and to specify the identity of female reproductive organs. In rice, the AG subfamily gene OsMADS13 is intimately involved in the determination of ovule identity, since knock-out mutant plants develop carpel-like structures in place of ovules resulting in female sterility. Little is known about the regulatory pathways at the base of rice gynoecium development. To further investigate molecular mechanisms acting downstream of OsMADS13, we obtained transcriptomes of immature inflorescences from wild type and the osmads13 mutant. Among a total of 476 Differentially Expressed Genes (DEGs), a substantial overlap with DEGs from the SEP-family osmads1 mutant was found, suggesting that OsMADS1 and OsMADS13 may act on a common set of target genes. Expression studies and preliminary analyses of two upregulated Zinc-finger transcription factor encoding genes indicate that our dataset represents a valuable resource for the identification of both OsMADS13 target genes and novel players in rice ovule development. Taken together, our study suggests that OsMADS13 is an important repressor of the carpel pathway during ovule development.
floral meristem determinacy; gynoecium; ovule; rice; transcription;
Settore BIO/18 - Genetica
Settore BIO/11 - Biologia Molecolare
9-ott-2020
JOURNAL OF EXPERIMENTAL BOTANY
Article (author)
File in questo prodotto:
File Dimensione Formato  
eraa460.pdf

embargo fino al 09/10/2021

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 1.59 MB
Formato Adobe PDF
1.59 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/777025
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 4
social impact