Bone remodeling and repair require osteogenic cells to reach the sites that need to be rebuilt, indicating that stimulation of osteoblast migration could be a promising osteoanabolic strategy. We showed that purified δ-tocotrienol (δ-TT, 10 µg/mL), isolated from commercial palm oil (Elaeis guineensis) fraction, stimulates the migration of both MC3T3-E1 osteoblast-like cells and primary human bone marrow mesenchymal stem cells (BMSC) as detected by wound healing assay or Boyden chamber assay respectively. The ability of δ-TT to promote MC3T3-E1 cells migration is dependent on Akt phosphorylation detected by Western blotting and involves Wnt/β-catenin signalling pathway activation. In fact, δ-TT increased β-catenin transcriptional activity, measured using a Nano luciferase assay and pretreatment with procaine (2 µM), an inhibitor of the Wnt/β-catenin signalling pathway, reducing the wound healing activity of δ-TT on MC3T3-E1 cells. Moreover, δ-TT treatment increased the expression of β-catenin specific target genes, such as Osteocalcin and Bone Morphogenetic Protein-2, involved in osteoblast differentiation and migration, and increased alkaline phosphatase and collagen content, osteoblast differentiation markers. The ability of δ-TT to enhance the recruitment of BMSC, and to promote MC3T3-E1 differentiation and migratory behavior, indicates that δ-TT could be considered a promising natural anabolic compound.
Food for bone : Evidence for a role for delta-tocotrienol in the physiological control of osteoblast migration / L. Casati, F. Pagani, R. Maggi, F. Ferrucci, V. Sibilia. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 21:13(2020 Jun 30), pp. 4661.1-4661.17.
|Titolo:||Food for bone : Evidence for a role for delta-tocotrienol in the physiological control of osteoblast migration|
CASATI, LAVINIA (Primo)
PAGANI, FRANCESCA (Secondo)
FERRUCCI, FRANCESCO (Penultimo)
SIBILIA, VALERIA (Ultimo) (Corresponding)
|Parole Chiave:||Delta-tocotrienol; H3 and H4 acetylation level; MC3T3-E1 cells; Osteoblast migration; Wnt/β-catenin pathway|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore MED/04 - Patologia Generale
Settore BIO/09 - Fisiologia
|Progetto:||Epigenetic in the pathophysiology of Osteoporosis: new insight from in vivo and in vitro studies.|
|Data di pubblicazione:||30-giu-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ijms21134661|
|Appare nelle tipologie:||01 - Articolo su periodico|