Eukaryotic cells treated with microtubule-targeting agents activate the spindle assembly checkpoint to arrest in mitosis and prevent chromosome mis-segregation. A fraction of mitotically arrested cells overcomes the block and proliferates even under persistent checkpoint-activating conditions. Here, we asked what allows proliferation in such unfavourable conditions. We report that yeast cells are delayed in mitosis at each division, implying that their spindle assembly checkpoint remains responsive. The arrest causes their cell cycle to be elongated and results in a size increase. Growth saturates at mitosis and correlates with the repression of various factors involved in translation. Contrary to unperturbed cells, growth of cells with an active checkpoint requires Cdh1. This peculiar cell cycle correlates with global changes in protein expression whose signatures partly overlap with the environmental stress response. Hence, cells dividing with an active checkpoint develop recognisable specific traits that allow them to successfully complete cell division notwithstanding a constant mitotic checkpoint arrest. These properties distinguish them from unperturbed cells. Our observation may have implications for the identification of new therapeutic windows and targets in tumors.
Cellular response upon proliferation in the presence of an active mitotic checkpoint / A. Corno, E. Chiroli, F. Gross, C. Vernieri, V. Matafora, S. Maffini, M. Cosentino Lagomarsino, A. Bachi, A. Ciliberto. - In: LIFE SCIENCE ALLIANCE. - ISSN 2575-1077. - 2:3(2019), pp. e201900380.1-e201900380.16.
|Titolo:||Cellular response upon proliferation in the presence of an active mitotic checkpoint|
|Parole Chiave:||Cell Cycle Proteins; Cell Division; Cell Proliferation; Cell Size; Gene Expression Profiling; Mitosis; Models, Biological; Mutation; Single-Cell Analysis; Transcriptome; Cell Cycle Checkpoints|
|Settore Scientifico Disciplinare:||Settore FIS/02 - Fisica Teorica, Modelli e Metodi Matematici|
Settore BIO/06 - Anatomia Comparata e Citologia
|Data di pubblicazione:||2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.26508/lsa.201900380|
|Appare nelle tipologie:||01 - Articolo su periodico|