Copy number variants (CNVs) are pervasive in several animal and plant genomes and contribute to shaping genetic diversity. In barley, there is evidence that changes in gene copy number underlie important agronomic traits. The recently released reference sequence of barley represents a valuable genomic resource for unveiling the incidence of CNVs that affect gene content and identifying sequence features associated with CNV formation. Using exome sequencing and read count data, we detected 16,605 deletions and duplications that affect barley gene content by surveying a diverse panel of 172 cultivars, 171 landraces, 22 wild relatives and other 32 uncategorized domesticated accessions. The quest for segmental duplications (SDs) in the reference sequence revealed many low-copy repeats, most of which overlap predicted coding sequences. Statistical analyses revealed that the incidence of CNVs increases significantly in SD-rich regions, indicating that these sequence elements act as hot spots for the formation of CNVs. This study delivers a comprehensive genome-wide study of CNVs affecting barley gene content and implicates SDs in the molecular mechanisms that lead to the formation of this class of CNVs.

Segmental duplications are hot spots of copy number variants affecting barley gene content / G. Bretani, L. Rossini, C. Ferrandi, J. Russell, R. Waugh, B. Kilian, P. Bagnaresi, L. Cattivelli, A. Fricano. - In: PLANT JOURNAL. - ISSN 0960-7412. - 103(2020 Aug), pp. 1073-1088. [10.1111/tpj.14784]

Segmental duplications are hot spots of copy number variants affecting barley gene content

G. Bretani
Primo
;
L. Rossini
Secondo
;
A. Fricano
Ultimo
2020

Abstract

Copy number variants (CNVs) are pervasive in several animal and plant genomes and contribute to shaping genetic diversity. In barley, there is evidence that changes in gene copy number underlie important agronomic traits. The recently released reference sequence of barley represents a valuable genomic resource for unveiling the incidence of CNVs that affect gene content and identifying sequence features associated with CNV formation. Using exome sequencing and read count data, we detected 16,605 deletions and duplications that affect barley gene content by surveying a diverse panel of 172 cultivars, 171 landraces, 22 wild relatives and other 32 uncategorized domesticated accessions. The quest for segmental duplications (SDs) in the reference sequence revealed many low-copy repeats, most of which overlap predicted coding sequences. Statistical analyses revealed that the incidence of CNVs increases significantly in SD-rich regions, indicating that these sequence elements act as hot spots for the formation of CNVs. This study delivers a comprehensive genome-wide study of CNVs affecting barley gene content and implicates SDs in the molecular mechanisms that lead to the formation of this class of CNVs.
Barley; Copy number variants; Exome sequencing; Segmental duplications
Settore AGR/07 - Genetica Agraria
   Wheat and barley Legacy for Breeding Improvement (WHEALBI)
   WHEALBI
   EUROPEAN COMMISSION
   FP7
   613556
ago-2020
27-apr-2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/732669
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