The growing interest in medicinal plants for the identification of new bioactive compounds and the formulation of new nutraceuticals and drugs prompted us to develop a powerful experimental approach allowing the detailed metabolic profiling of complex plant extracts, the identification of ligands of macromolecular targets of biomedical relevance and a preliminary characterization of their biological activity. To this end, we selected Peucedanum ostruthium, a plant traditionally employed in Austria and Italy for its several potential therapeutic applications, as case study. We combined the use of NMR and UPLC-HR-MS for the identification of the metabolites present in its leaves and rhizome extracts. Due to the significant content of polyphenols, particularly chlorogenic acids, recently identified as anti-amyloidogenic compounds, polyphenols-enriched fractions were prepared and tested for their ability to prevent Aβ1-42 peptide aggregation and neurotoxicity in a neuronal human cell line. STD-NMR experiments allowed the detailed identification of Aβ oligomers’ ligands responsible for the anti-amyloidogenic activity. These data provide experimental protocols and structural information suitable for the development of innovative molecular tools for prevention, therapy and diagnosis of Alzheimer's disease.
bioNMR-based identification of natural anti-Aβ compounds in Peucedanum ostruthium / A. Palmioli, S. Bertuzzi, A. De Luigi, L. Colombo, B. La Ferla, M. Salmona, I. De Noni, C. Airoldi. - In: BIOORGANIC CHEMISTRY. - ISSN 0045-2068. - 83(2019 Mar), pp. 76-86.
|Titolo:||bioNMR-based identification of natural anti-Aβ compounds in Peucedanum ostruthium|
PALMIOLI, ALESSANDRO (Corresponding)
|Parole Chiave:||Alzheimer's disease; Anti-amyloidogenic compounds; Chlorogenic acids; Furanocoumarins; NMR spectroscopy; Peucedanum ostruthium; UPLC-HR-MS; Amyloid beta-Peptides; Apiaceae; Biological Products; Dose-Response Relationship, Drug; Molecular Structure; Plant Extracts; Plant Leaves; Structure-Activity Relationship; Nuclear Magnetic Resonance, Biomolecular|
|Settore Scientifico Disciplinare:||Settore AGR/15 - Scienze e Tecnologie Alimentari|
|Data di pubblicazione:||mar-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bioorg.2018.10.016|
|Appare nelle tipologie:||01 - Articolo su periodico|
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