An in vitro approach was performed to assess the quality of drinking water collected at two treatment/ distribution networks located near the source (Plant #1) and the mouth of River Po (Plant #2). The water was sampled at different points of each distribution network, before (raw water) and after the chlorine dioxide disinfection, and in two points of the pipeline system to evaluate the influence of the distribution system on the amount and quality of the disinfection by-product. Cytotoxicity and genotoxicity of water extracts were evaluated in human peripheral lymphocytes and Hep-G2 cells by the use of the micronucleus (MN) test and Comet assay. Raw water samples of both plants induced cytotoxic effects, but not the increases of MN frequency in Hep-G2 cells and in human lymphocytes. Increases of DNA damage in human leukocytes was detected by Comet assay for raw water of Plant #2 at concentration ≥0.25 Leq/mL. The disinfection process generally has reduced the toxicity of water samples, even if potential direct DNA-damaging compounds have been detectable in drinking water samples. The proposal approach, if currently used together with chemical analysis, can contribute to improve the monitoring drinking water.
Drinking water quality: An in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system / F. Maffei, F. Carbone, G.C. Forti, A. Buschini, P. Poli, C. Rossi, L. Marabini, S. Radice, E. Chiesara, P. Hrelia. - In: ENVIRONMENT INTERNATIONAL. - ISSN 0160-4120. - 35:7(2009), pp. 1053-1061.
|Titolo:||Drinking water quality: An in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system|
CHIESARA, ENZO (Penultimo)
|Parole Chiave:||Comet assay; Disinfection by-product; Drinking water; Health risk; Micronucleus test|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.envint.2009.05.007|
|Appare nelle tipologie:||01 - Articolo su periodico|